Fracture resistance of simulated immature maxillary anterior teeth restored with various canal filling materials, with micro-posts or with a fiber post.

The aim of this in vitro study was to compare the fracture resistance of immature anterior teeth restored with different biomaterials. After preparation of bovine incisors to simulate immature permanent teeth, 98 specimens were assigned into seven groups as follows: G1 -negative control group; G2 -apexification with hydraulic calcium silicate cement in the whole root; G3 -apexification and gutta percha; G4 -apexification and fiber post; G5 -apexification with micro-posts; G6 -apexification with composite; and G7 -revascularization. Both the fracture resistance and the location where the fracture occurred were recorded.

In vitro phenotypic effects of Lipoxin A4 on M1 and M2 polarized macrophages derived from THP-1.

Background: Lipoxin A4 (LXA4) is a specialized pro-resolving mediator involved in the resolution phase of inflammation that is crucial for the return of tissues to homeostasis, healing, and regenerative processes. LXA4 can modify the microenvironment via its receptor, formyl peptide receptor 2 (FPR2) and thus modulate the inflammatory response. However, the effect of exogeneous LXA4 application on polarized macrophages remains unstudied.

Knowledge and Opinions of French Dental Students in Operative Dentistry - Management of Deep Carious Lesions.

Purpose: A questionnaire survey was recently undertaken among French dental students (FDSs) to investigate their practices, knowledge and opinions in various domains of minimal intervention (MI) in cariology. The present work focuses on management of deep carious lesions (DCLs).

Materials And Methods: The questionnaire was administered (Spring 2018) to all the fifth-year students of the 16 French dental schools.

Specialized pro-resolving lipid mediators in endodontics: a narrative review.

Endodontics is the branch of dentistry concerned with the morphology, physiology, and pathology of the human dental pulp and periradicular tissues. Human dental pulp is a highly dynamic tissue equipped with a network of resident immunocompetent cells that play major roles in the defense against pathogens and during tissue injury. However, the efficiency of these mechanisms during dental pulp inflammation (pulpitis) varies due to anatomical and physiological restrictions.

Transforaminal and systemic diffusion of an active agent from a zinc oxide eugenol-based endodontic sealer containing hydrocortisone-in an in vivo model.

Objectives: This study aimed to quantify in vivo the release of hydrocortisone acetate (HCA) contained in a zinc oxide eugenol-based endodontic sealer, in various tissues.

Materials And Methods: Roots of human teeth, shaped with One Shape single file and sealed with Endomethasone N, previously radiolabelled with tritium (H-HCA), were implanted in the back of 24 mice. Mice were sacrificed at 2, 8, 24, and 48 h to evaluate and quantify the amount of radioactivity in subcutaneous tissues surrounding the apex (periapical-like) of the implanted teeth, blood, spleen, kidneys, liver, and urine.

Autosomal recessive Treacher Collins syndrome due to POLR1C mutations: Report of a new family and review of the literature.

Treacher Collins syndrome (TCS) is a frequent cause of mandibulofacial dysostosis. To date, TCS-causing mutations in three genes, namely TCOF1, POLR1D, and POLR1C have been identified. TCS is usually inherited in an autosomal dominant manner, with a high clinical variability and no phenotype-genotype correlation.

The Effect of Magnification Loupes on Spontaneous Posture Change of Dental Students During Preclinical Restorative Training.

Scientific evidence validating the beneficial effect of loupes in preventing musculoskeletal disorders is very scarce. The aim of this study was to assess the impact of dental loupes on dental students' posture during a preclinical restorative dentistry course. Using a randomized crossover design, this study was conducted at the School of Dentistry, University of Nantes, France, in 2017.

Enhancement of the biocompatibility by surface nitriding of a low-modulus titanium alloy for dental implant applications.

To enhance their longevity, dental implants must be highly biocompatible and must have a low elastic modulus close to that of the bone. They must also possess a high superficial hardness and a high corrosion resistance. For these reasons, a recently developed low-modulus Ti-27Nb alloy with nontoxic elements was treated by gas nitriding at high temperature in this study.

Spheroid model study comparing the biocompatibility of Biodentine and MTA.

The primary objective of this study was to assess the biological effects of a new dentine substitute based on Ca₃SiO₅ (Biodentine™) for use in pulp-capping treatment, on pseudo-odontoblastic (MDPC-23) and pulp (Od-21) cells. The secondary objective was to evaluate the effects of Biodentine and mineral trioxide aggregate (MTA) on gene expression in cultured spheroids. We used the acid phosphatase assay to compare the biocompatibility of Biodentine and MTA.

In vitro effects of Choukroun's platelet-rich fibrin conditioned medium on 3 different cell lines implicated in dental implantology.

Objective: The aim of this work was to determine the relevance of Choukroun's platelet-rich fibrin (PRF) in dental implantology by determining the in vitro effects of soluble factors released by PRF clot. We used 3 different cell lines implicated in dental implantology: osteoblast, keratinocyte, and fibroblast.

Methods: Cellular viability, cell proliferation, and gene expression were analyzed using PRF conditioned medium.

Effect of hydroxyapatite and beta-tricalcium phosphate nanoparticles on promonocytic U937 cells.

Objectives: Nanoparticles from implanted materials are reported as the main cause of implant failure. Monocytes are among the first cells to colonize the inflammatory site. We evaluated the biological effects of bone substitutes presented to U937 cells in vitro as micron- or nanometer-sized particles.

Effect of three nickel-titanium rotary file techniques on infected root dentin reduction.

Objective: This study compared the bacterial reduction of in vitro infected root canals after instrumentation by 3 nickel-titanium (NiTi) rotary files with different taper and diameter versus manual stainless steel files.

Study Design: Sixty-four single-rooted human teeth were infected with a suspension of Streptococcus sanguis measured by optical density. Teeth were divided randomly into 4 groups of 16 and prepared with Flexofiles, GT rotary files, HERO 642, and ProFile.

Effect of a low-concentration EDTA solution on root canal walls: a scanning electron microscopic study.

Objectives: The aim of this study was to evaluate, in vitro, the ability of an 8% EDTA solution to remove debris and smear produced during root canal preparation.

Study Design: Single-rooted human teeth were used for this experiment. All root canals were instrumented in a similar fashion and irrigated with a 3.