Publications by authors named "Fabiana Munhoz"
Polarization-resolved coherent anti-Stokes Raman scattering (CARS) is usually applied to measure the depolarization ratio in solutions or evidence orientation effects in anisotropic media. We present an extensive approach based on multiple-field polarization-resolved CARS, in order to unravel the complexity of vibrational resonances up to the fourth-order symmetry, at the microscopic scale in nonisotropic media. The CARS polarized signals measured under a continuous variation of the incident pump and/or Stokes excitation beams are analyzed using a full tensorial picture both in the nonresonant and resonant regimes.
View Article and Find Full Text PDFArticle Synopsis
- The study focuses on how the anisotropic properties of biological and molecular samples affect polarization resolved nonlinear microscopy imaging.
- It highlights that birefringence can negatively impact methods like Second Harmonic Generation (SHG) and Two-Photon Excited Fluorescence (TPEF), potentially leading to inaccurate sample analysis.
- The authors suggest a new approach to measure birefringence and properly consider its effects during experiments to improve the accuracy of the imaging results.
Article Synopsis
- This study examines how various experimental factors impact polarization responses in two-photon inverted microscopy, focusing on the effects of reflection optics and high numerical aperture focusing.
- The researchers found that these factors can distort polarization responses, potentially leading to inaccurate readings of molecular order from polarimetric measurements.
- A new model is proposed to account for these distortions, along with a calibration technique for accurately determining polarization parameters in sample liquids during two-photon fluorescence polarimetry.