Publications by authors named "Fabian Rieder"
Article Synopsis
- Fluorescing proteins that emit in the near-infrared region, particularly engineered phytochromes, are vital for biomedicine and life sciences, enabling in vivo monitoring of biological systems.
- The study focuses on two minimal phytochromes, miRFP670nano3 and miRFP718nano, examining how their structural characteristics affect the fluorescence of the linked biliverdin chromophore.
- Results indicate that the biliverdin is deprotonated at specific pyrrole rings in both proteins, with differing rates of proton exchange due to their structural dynamics, ultimately influencing their fluorescent efficiency.
The pH dependence of the absorption and (time-resolved) fluorescence of two red-shifted fluorescent proteins, mCardinal and mNeptune, was investigated. Decay-associated spectra were measured following fluorescence excitation at 470 nm in PBS buffer with a pH that ranged from 5.5 to 8.
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