Development of MHz X-ray phase contrast imaging at the European XFEL.

We report on recent developments that enable megahertz hard X-ray phase contrast imaging (MHz XPCI) experiments at the Single Particles, Clusters, and Biomolecules and Serial Femtosecond Crystallography (SPB/SFX) instrument of the European XFEL facility (EuXFEL). We describe the technical implementation of the key components, including an MHz fast camera and a modular indirect X-ray microscope system based on fast scintillators coupled through a high-resolution optical microscope, which enable full-field X-ray microscopy with phase contrast of fast and irreversible phenomena. The image quality for MHz XPCI data showed significant improvement compared with a pilot demonstration of the technique using parallel beam illumination, which also allows access to up to 24 keV photon energies at the SPB/SFX instrument of the EuXFEL.

Optic generation and perpetuation of acoustic bubble clusters.

Laser-induced cavitation bubbles offer precise control of the flow in space and time, but they are rarely used for the mechanical and chemical processing of liquids. Instead, strong acoustic fields are commonly used to nucleate and drive cavitation bubbles for liquid process applications. While acoustic field creates many more cavitation events, the resulting chaotic dynamics offers little control on the fluid mechanics, i.

Amplification of Supersonic Microjets by Resonant Inertial Cavitation-Bubble Pair.

We reveal for the first time by experiments that within a narrow parameter regime, two cavitation bubbles with identical energy generated in antiphase develop a supersonic jet. High-resolution numerical simulation shows a mechanism for jet amplification based on toroidal shock wave and bubble necking interaction. The microjet reaches velocities in excess of 1000  m s^{-1}.

Shear wave generation from non-spherical bubble collapse in a tissue phantom.

Elastography is a non-invasive technique to detect tissue anomalies the local elastic modulus using shear waves. Commonly shear waves are produced acoustic focusing or the use of mechanical external sources, shear waves may result also naturally from cavitation bubbles during medical intervention, for example from thermal ablation. Here, we measure the shear wave emitted from a well-controlled single laser-induced cavitation bubble oscillating near a rigid boundary.

Integrated biophysical matching of bacterial nanocellulose coronary artery bypass grafts towards bioinspired artery typical functions.

Revascularization via coronary artery bypass grafting (CABG) to treat cardiovascular disease is established as one of the most important lifesaving surgical techniques worldwide. But the shortage in functionally self-adaptive autologous arteries leads to circumstances where the clinical reality must deal with fighting pathologies coming from the mismatching biophysical functionality of more available venous grafts. Synthetic biomaterial-based CABG grafts did not make it to the market yet, what is mostly due to technical hurdles in matching biophysical properties to the complex demands of the CABG niche.

In situ measurement of cavitation damage from single bubble collapse using high-speed chronoamperometry.

We quantitatively study cavitation damage non-invasively, in-place and time-resolved at microsecond resolution. A single, laser-induced bubble is generated in an aqueous NaCl solution close to the surface of an aluminum sample. High-speed chronoamperometry is used to record the corrosion current flowing between the sample and an identical aluminum electrode immersed in the same solution.

Cavitation erosion by shockwave self-focusing of a single bubble.

The ability of cavitation bubbles to effectively focus energy is made responsible for cavitation erosion, traumatic brain injury, and even for catalyse chemical reactions. Yet, the mechanism through which material is eroded remains vague, and the extremely fast and localized dynamics that lead to material damage has not been resolved. Here, we reveal the decisive mechanism that leads to energy focusing during the non-spherical collapse of cavitation bubbles and eventually results to the erosion of hardened metals.

Multi-frame multi-exposure shock wave imaging and pressure measurements.

Shock wave visual detection was traditionally performed using streak cameras, limited to homogeneous shock wave emission, with the corresponding shock wave pressure measurements available at rather large distances or numerically estimated through equation of state for water. We demonstrate a multi-frame multi-exposure shock wave velocity measurement technique for all in-plane directions of propagation, based on custom-built illumination system allowing multiple illumination pulses within each frame at multi-MHz frame rates and at up to 200 MHz illumination pulse repetition frequency at sub-nanosecond pulse durations. The measurements are combined and verified using a fiber-optic probe hydrophone, providing independent shock wave pressure and time-of-flight measurements, creating a novel all-optical measurement setup.

Corrosion and material alterations of a CuZn38Pb3 brass under acoustic cavitation.

An alloy that is exposed to cavitation may experience mechanical cavitation damages as well as accelerated corrosion. In the present paper, the evolution of corrosion erosion behavior of brass samples (CuZn38Pb3) during continuous exposure to ultrasonic cavitation in a salt solution (NaCl) was investigated. Various samples were sonicated for times between 0 min and 5 h.

Bubble size measurements in different acoustic cavitation structures: Filaments, clusters, and the acoustically cavitated jet.

Acoustic cavitation typically forms a variety of bubble structures of generally unknown and broad size distributions. As the bubbles strongly oscillate, their (equilibrium) sizes are not directly observable. Here, a method is presented to experimentally determine the size distribution in bubble populations from high-speed imaging of the bubbles in oscillation.

Membrane cleaning with ultrasonically driven bubbles.

A laboratory filtration plant for drinking water treatment is constructed to study the conditions for purely mechanical in situ cleaning of fouled polymeric membranes by the application of ultrasound. The filtration is done by suction of water with defined constant contamination through a membrane module, a stack of five pairs of flat-sheet ultrafiltration membranes. The short cleaning cycle to remove the cake layer from the membranes includes backwashing, the application of ultrasound and air flushing.

Vortex dynamics of collapsing bubbles: Impact on the boundary layer measured by chronoamperometry.

Cavitation bubbles collapsing in the vicinity to a solid substrate induce intense micro-convection at the solid. Here we study the transient near-wall flows generated by single collapsing bubbles by chronoamperometric measurements synchronously coupled with high-speed imaging. The individual bubbles are created at confined positions by a focused laser pulse.

Mechanisms of single bubble cleaning.

The dynamics of collapsing bubbles close to a flat solid is investigated with respect to its potential for removal of surface attached particles. Individual bubbles are created by nanosecond Nd:YAG laser pulses focused into water close to glass plates contaminated with melamine resin micro-particles. The bubble dynamics is analysed by means of synchronous high-speed recordings.

Quantitation of major protein constituents of murine intestinal fluid.

The gastrointestinal tract is a hostile biological environment, yet not all ingested materials are destroyed. The minute differences that determine whether a substance persists or is digested, liberated, adsorbed, excreted, or taken up are still poorly understood. Most attempts to investigate the events occurring during an orogastrointestinal passage rely on simplified in vitro systems where an analyte is exposed to artificial intestinal fluids.

Bystander protein protects potential vaccine-targeting ligands against intestinal proteolysis.

Endowing mucosal vaccines with ligands that target antigen to mucosal lymphoid tissues may improve immunization efficacy provided that the ligands withstand the proteolytic environment of the gastro-intestinal tract until they reach their destination. Our aim was to investigate whether and how three renowned ligands - Ulex europaeus agglutinin I and the B subunits of cholera toxin and E. coli heat-labile enterotoxin - master this challenge.