Unraveling multistate unfolding of pig kidney fructose-1,6-bisphosphatase using single tryptophan mutants.

Pig kidney fructose-1,6-bisphosphatase is a homotetrameric enzyme which does not contain tryptophan. In a previous report the guanidine hydrochloride-induced unfolding of the enzyme has been described as a multistate process [Reyes, A. M.