[Development of a rapid test kit for antibody to HIV by nano immunomagnetic lateral flow method].

Objective: To develop a rapid test kit for antibody to HIV by nano immunomagnetic lateral flow method.

Methods: A rapid test kit was developed by conjugation of the HIV antigen gp41 and gp36 to 200nm super paramagnetic particles by carbodiimide (EDC) and coating of the HIV antigen gp41 and gp36 to nitrocellulose membrane. Then the kit was evaluated with serials of experiments.

[Effects of stress on the cognitive function and physical fitness and its biological mechanisms].

Aim: To study the effects of stress on the cognitive function and physical fitness and its biological mechanisms, provide scientific basis for seeking protective measures to reduce stress-induced damage.

Methods: The model of restraint stress was adopted in our experiment. Step-down test and exhaustive swimming test were used to measure the learning and memory function and physical fitness in mice, respectively.

[Differentially expressed genes associated with cold acclimation].

To investigate the upregulated genes associated with cold acclimation, a cold acclimation model was established based on Balb/C mouse. mRNA of muscle and liver were isolated, and the upregulated genes of these tissues were studied by representational differential analysis (RDA). The upregulated genes then were sequenced and searched by Blast software in GenBank database.