Prevalence of HCV Antibody and its Associated Factors: A Study from Sentinel Hospitals in China.

Objective: The prevalence and related factors of serum anti-HCV in different regions and hospitals have not been studied extensively in China. We used routine screening data to determine the prevalence of HCV antibody in hospital patients, evaluate the epidemic trend of hepatitis C and formulate screening strategies.

Methods: Patient information and HCV antibody testing results were collected from January 2017 to December 2019 in 77 HCV sentinel hospitals in China.

[Situation on HIV/AIDS epidemics among migrant population in China, 2008 - 2011].

Objective: To analyze the HIV/AIDS epidemics situation among migrant population.

Methods: Data on HIV/AIDS cases among mobile population through case reporting system was collected from 2008 to 2011.

Results: The number of reported cases on HIV/AIDS among mobile population had been rising, with proportions of the total reported cases increased from 10.

[Epidemic characteristics of HIV/AIDS among men who have sex with men from 2006 to 2010 in China].

Objective: To analyze the epidemic characteristics of HIV/AIDS among men who have sex with men (MSM) from 2006 to 2010 in China, and to provide evidence for the development of prevention and control measures.

Methods: Through the network reporting information system. The distribution of MSM transmission was analyzed.

[Study on the HIV infection status, characteristics of AIDS-related behaviors and risk factors among men attending the sexually transmitted disease clinics].

Objective: To understand the status on AIDS awareness, AIDS-related behaviors, risk factors on HIV infection status among 15 - 90 years or older men at the sexually transmitted disease clinics.

Methods: Data from the 2009 and 2010 national sentinel surveillance system, regarding men attending sexually transmitted disease clinics was collected from Guangdong, Guangxi, Henan, Sichuan, Yunnan and Jiangxi provinces, where the AIDS epidemic among 15 - 90 years or older population was serious. Data was uploaded to National Center for AIDS/STD Control and Prevention (NCAIDS) through the comprehensive AIDS control and prevention information system.

[HIV/AIDS epidemic situation and data analysis among young students from 2006-2009 in China].

Objective: To analyze the HIV/AIDS epidemic situation and related behavioral components among young students in the last years and to provide information for integrated control and prevention programs.

Methods: Data on young students (15 - 24 years) through case reporting system was collected from 2006 to 2009 and continuous sentinel surveillance data regarding 6 national sentinel sites carried out from 2006 to 2009 was also collected. The sentinel sites would include universities, junior college and secondary vocational colleges registered students, using cluster sampling phases method.

[Data analysis of national HIV comprehensive surveillance sites among female sex workers from 2004 to 2008].

Objective: To monitor the time trend of behaviors change related to HIV/AIDS epidemic and HIV prevalence, and to provide information and basis for comprehensive HIV prevention responses and evaluation of intervention effectiveness among female sex workers in China.

Methods: During the year of 2004 to 2008, a series of cross-sectional surveys were conducted among female sex workers in community recruited in both entertainment places and in street, during the defined timeframe, a face-to-face interview was conducted with uniformed questionnaire; while, the venous blood specimen was collected. The sample size was 400 per site per survey.

[Viral load test conducive to excluding negative subjects from suspects in HIV antibody detections].

Objective: To study whether plasma viral load testing is helpful to exclude ones free from Human immunodeficiency virus (HIV) infections from suspects in HIV antibody detections.

Methods: 19 Specimens, which showed disconcordant results of the two HIV EIA testing (S/CO < 6) and indeterminated results of Western blot (WB) test, were selected. Viral load of the specimens were detected.

Field evaluation of alternative testing strategies for the detection of HIV infection in Beijing.

Objective: To identify a cost-efficient alternative antibody testing strategy for screening and confirmation of HIV infection by rapid simple tests (RSTs) and enzyme-linked immunosorbent assays (ELISAs).

Methods: Four RSTs (RST1, RST2, RST3, and RST4) and five ELISAs (ELISA1, ELISA2, ELISA3, ELISA4, and ELISA5) were evaluated in two phases by using banked and serum specimens prospectively collected at regional hospitals and voluntary counseling and testing (VCT) centers in Beijing. A total of 200 banked serum specimens were included in the first phase, including 62 HIV-positive, 127 HIV-negative and 11 indeterminate specimens.

[Subtype and sequence analysis of gag and env genes among HIV-1 strains circulating in Beijing residents during 2006].

Objective: To investigate the subtype distribution and the prevalence of sequence characteristics of HIV-1 strains in Beijing residents during 2006 and to analyze the relationship between distribution of HIV-1 subtypes and transmission routines.

Methods: Blood samples from 32 new confirmed HIV-1 infected individuals from Beijing residents in 2006 and separated plasma specimens were collected. RNAs were extracted and the gag and env gene were amplified by RT-PCR and nest-PCR.

Association of neutralization sensitivity of HIV-1 primary isolates with biological properties of isolates from HIV-1 infected Chinese individuals.

Objective: Although HIV-1 infection is prevalent in many regions in China, it remains largely unknown on the biological characteristics of dominant circulating isolates. This study was designed to isolate the circulating viral strains from different prevalent regions and to characterize their biological properties and neutralization sensitivity.

Methods: Primary viruses were isolated from fresh PBMCs using the traditional co-culture method and their capacity of inducing syncytium was tested in MT-2 cells.

[Biological characteristics of HIV-1 isolates circulating in China are linked to its env V3 loop sequence variability].

Objective: To investigate the biological characteristics of the HIV-1 isolates circulating in China and to define the association of these properties with env V3 loop sequence variability.

Methods: Primary viruses were isolated from fresh peripheral blood mononuclear cells (PBMCs) using the traditional co-culture method and their capacity of inducing syncytium was tested in MT-2 cells; meanwhile, their coreceptor usage was determined with GHOST-cell lines which stably express CD4 and the chemokine receptor CCR5 or CXCR4. Furthermore, HIV-1 V3 and its flanking region sequences were amplified by nest-polymerase chain reaction (nest-PCR) and sequenced.

[Sequence analysis of gag-pol gene of HIV-1 B/C recombinant viruses in China].

Objective: To characterize full length gag gene and partial pol gene of Chinese prevalent HIV-1 B/C recombinant strains, to explore the genetic difference between parent strains and B/C recombinant strains, and to investigate the mechanism of different biologic phenotype among them.

Methods: The peripheral blood samples were collected from 138 HIV-positive persons from 12 regions of China respectively. Samples of total DNA were extracted from the peripheral blood mononuclear cells to undergo nested PCR and sequencing.