Publications by authors named "Fa-Jie Feng"
Article Synopsis
- Rehmannia glutinosa is a valuable traditional Chinese medicine, but it faces cultivation challenges due to severe continuous cropping obstacles, requiring land to be left fallow for several years.
- MicroRNAs (miRNAs) are small RNA molecules that help regulate plant physiological activities, particularly in response to environmental stress, by targeting specific genes.
- The article discusses the importance of researching miRNAs in R. glutinosa to better understand and address its cultivation issues and outlines future trends in this field of study.
[Advances on molecular mechanisms of Rehmannia glutinosa consecutive monoculture problem formation in multi-omics era].
Zhongguo Zhong Yao Za Zhi
February 2017
Article Synopsis
- - The paper discusses the unresolved issues with consecutive monoculture problems, highlighting the complex interactions in the rhizosphere soil as significant factors leading to these issues.
- - It emphasizes the role of cultivated medicinal plants, toxic allelochemicals, and rhizosphere microbes as key contributors to these problems, using R. glutinosa as a case study.
- - The authors advocate for the use of 'omics' technologies, like genomics and metabolomics, to better understand and address the molecular mechanisms underlying consecutive monoculture challenges.
Objective: To study the allelopathic potential of Rehmannia glutinosa root exudates in different growth stages and dynamic change of phenolic acids contents, in order to reveal the correlation between phenolic acids and allelbpathy effect of Rehmannia glutinosa.
Methods: Root exudates of Rehmannia glutinosa in different growth stages were obtained by a new instrument which was used to collect the root exudates of xerophytes. After that, bioassay was applied to estimate allelopathy effect of the root exudates.
Using cDNA from Rehmannia glutinosa leaf as template, a 972 bp fragment of expansin gene which containing a 762 bp ORF that encoded 253 amino acids, was cloned, named RgEXPA10, which GenBank accession number for this gene is KF011918. A 1 207 bp genomic sequence of RgEXPA10 was amplified by PCR with leaf DNA as template, sequencing analysis revealed that three exons and two introns in RgEXPA10 genomic sequence, and which GenBank accession number is KF011919. Molecular and bioinformatic analyses indicated that RgEXPA10 protein have DPBB_1 and Pollen_allerg_1 domain, also including a 26 aa nuclear localization signal and a 19 aa transmembrane region.
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