Homogenate-assisted high-pressure disruption extraction for determination of phenolic acids in Lonicerae Japonicae Flos.

An effective method based on the combined homogenate-assisted high-pressure disruption extraction (HHPDE) was applied to the extraction and determination of the main phenolic acid compounds from Lonicerae Japonicae Flos. The optimized HHPDE showed competitive advantage in yield (The extraction yields of NCA, CA, 3,5DCA and 4,5DCA in HHPDE were 1.21, 1.

Qualitative and Quantitative Characterization of Monosaccharide Components of , Liguspyragine Hydrochloride, and Glucose Injection.

, liguspyragine hydrochloride, and glucose injection (SLGI) was made of Salvia miltiorrhiza Bge., liguspyragine hydrochloride, glucose, and glycerin. There were many kinds of monosaccharide components in SLGI, which might be from the raw material and Bge.

Ganoderin A, a novel 9,11-secosterol from Ganoderma lucidum spores oil.

In this study, four sterols were isolated from the Ganoderma lucidum spores oil obtained via supercritical CO extraction. Four chemical constituents were ganoderin A (1), chaxine B (2), ergosterol, (3) and stellasterol (4). All the separated ingredients were characterized using spectral data interpretation and by comparing with reported data.

[Extraction of 10-Deacetyl Baccatin by Supercritical CO2 from Taxus yunnanensis Branches and Leaves].

Objective: To study the supercritical CO2 fluids extraction (SFE) method to extract the components from Taxus yunnanensis.

Methods: Medicinal meterials were extracted by supercritical CO2, and then purified by industrial chromatography. Using the extraction yield of 10-DAB as the index,single factor test was carried out to investigate the effect of co-solvent, extraction time, extraction pressure, extraction temperature, pressure and temperature of separation kettle I.

[Fingerprint and Simultaneous Determination of Multi-components in Water-soluble Components of Salvia miltiorrhiza in Miao Autonomous County of Songtao, Guizhou].

Objective: To establish the HPLC fingerprint of water-soluble components of Salvia miltiorrhiza in Songtao, Guizhou, and to perform simultaneous determination of six components in it, so as to provide analytical method for its quality control.

Methods: The analyses were performed on a Phenomenex Luna C18 (2) (250 mm x 4. 6 mm, 5µm) column eluted with 0.

[Study on Chemical Constituents of Fat-soluble Extraction from Lepidium meyenii].

Objective: To study the chemical constituents of the fat-soluble extraction from Lepidium meyenii root.

Methods: Different extraction methods were studied, including supercritical carbon dioxide extraction, circumfluence extraction and steam distillation. Chemical constituents of the fat-soluble extraction from Lepidium meyenii were analyzed by GC/MS.

[Chemical components from essential oil of Pandanus amaryllifolius leaves].

Objective: To analyze the chemical compositions of Pandanus amaryllifolius leaves essential oil extracted by steam distillation.

Methods: The essential oil of Pandanus amaryllifolius leaves was analyzed by gas chromatography-mass spectrum, and the relative content of each component was determined by area normalization method.

Results: 128 peaks were separated and 95 compounds were identified, which weighed 97.

[Supercritical CO2 extraction and component analysis of Aesculus wilsonii seed oil].

Objective: To research the optimal extraction process of supercritical CO2 extraction and analyze the component of the oil extracted from Aesculus wilsonii seed.

Methods: Using the yield of Aesculus wilsonii seed oil as the index, optimized supercritical CO2 extraction parameter by orthogonal experiment methodology and analysed the compounds of Aesculus wilsonii seed oil by GC-MS.

Results: The optimal parameters of the supercritical CO2 extraction of the oil extracted from Aesculus wilsoniit seed were determined: the extraction pressure was 28 MPa and the temperature was 38 degrees C, the separation I pressure was 12 MPa and the temperature was 40 degrees C, the separation II pressure was 5 MPa and the temperature was 40 degrees C, the extraction time was 110 min.

[Study on the immunocompetence of polysaccharide extracted from root of Salvia miltiorrhiza].

Objective: To study the immune activity of polysaccharide extracted from root of Salvia miltiorrhiza.

Methods: Investigated the effects of polysaccharide extracted from root of Salvia miltiorrhiza on lymphocyte proliferation response of mouse induced by LPS (the lipopolysaccharide LPS), phagocytosis of the peritoneal macrophage of mice to chick erythrocytes and the mouse models of delayed type hypersensitivity (DTH) response induced by DNFB.

Results: Lymphocyte proliferation and phagocytosis of the peritoneal macrophage of mice could be promoted by the polysaccharide, which could inhibit ear edema and capillary permeability increase induced by DNFB and enlarged the thymus and splenic index in mice.

[Optimization for supercritical CO2 extraction with response surface methodology of Prunus armeniaca oil].

Objective: To optimize the extraction conditions of Prunus armeniaca oil by Supercritical CO2 extraction and identify its components by GC-MS.

Methods: Optimized of SFE-CO extraction by response surface methodology and used GC-MS to analysis Prunus armeniaca oil compounds.

Results: Established the model of an equation for the extraction rate of Prunus armeniaca oil by supercritical CO2 extraction, and the optimal parameters for the supercritical CO2 extraction determined by the equation were: the extraction pressure was 27 MPa, temperature was 39 degrees C, the extraction rate of Prunus armeniaca oil was 44.

[Optimization for supercritical CO2 extraction with response surface methodology and component analysis of Sapindus mukorossi oil].

Objective: To study the extraction conditions of Sapindus mukorossi oil by Supercritical CO2 Extraction and identify its components.

Methods: Optimized SFE-CO2 Extraction by response surface methodology and used GC-MS to analysie Sapindus mukorossi oil compounds.

Results: Established the model of an equation for the extraction rate of Sapindus mukorossi oil by Supercritical CO2 Extraction, and the optimal parameters for the Supercritical CO2 Extraction determined by the equation were: the extraction pressure was 30 MPa, temperature was 40 degrees C; The separation I pressure was 14 MPa, temperature was 45 degrees C; The separation II pressure was 6 MPa, temperature was 40 degrees C; The extraction time was 60 min and the extraction rate of Sapindus mukorossi oil of 17.

[Research of the essential oil of Plumeria rubra var. actifolia from Laos by supercritical carbon dioxide extraction].

The orthogonal test and the supercritical carbon dioxide fluid extraction were used for optimizing the extraction of the essential oil from Plumeria rubra var. actifolia for the first time. Compared with the steam distillation, the optimal operation parameter of extraction was as follows: extraction pressure 25 MPa, extraction temperature 45 degrees C; separator I pressure 12 MPa, separator I temperature 55 degrees C; separator II pressure 6 MPa, separator II temperature 30 degrees C.

[Purification of Ganoderma lucidum polysaccharide by ultrafiltration technology].

Objective: To investigate the feasibility and technology for purifying Ganodermna lucidum polysaccharide (GLP) by ultrafiltration.

Methods: A spire roll ultrafiltration unit was selected to purify GLP.

Results: Purity of 73.

[Study on isolation of Chlorogenic acid from Eucommia ulmoides leaves by ultrafiltration technique].

Objective: To isolate and purify Chlorogenic acid from Eucommia ulmoides leaves by ultrafiltration technique.

Methods: Chlorogenic acid transferring ratio was investigated by temperature, pressure, time, cut-off molecular mass.

Results: The optimal conditions for the ultrafiltration technique were finally determined.

[Study on the technique of ultrasonic wave extraction of triterpeneclucoside].

The extraction technique of ultrasonic wave on increasing the triterpeneclucoside was studied. Based on single factor experiments and the orthogonal design introduced,the optimized extracting conditions of triterpeneclucoside were investigated. The results showed that the best extraction technique conditons are 65% alcohol-water, the ratio of solid to liquid 1:30,dousing over 30 minutes,ultrasonic wave lasting for 40 minutes, ultrasonic frequency 26.

[Study on supercritical CO2 extraction of fatty oils from the seed of Akebia trifoliata (Thunb) Koidz].

The extraction of fatty oils from the seed of Akebia trifoliata (Thunb) Koidz with supercritical CO2 was studied. The effects of extraction pressure, extraction temperature on the yields were discussed. The optimal condition of this method was as follow: extraction pressure 30 MPa, extraction temerature 45 degrees C, separator I pressure 11 MPa, separator I temperature 50 degrees C, separator II pressure 6MPa, separator II temperature 45 degrees C, extraction period 2 hours.

[Study on ingredients of essential oils of Curcuma wenyujin extracted by supercritical-CO2 fluid extraction and steam distillation].

Objective: To compare the ingredients of essential oils of Curcuma wenyujin extracted by supercritical-CO2 fluid extraction and by steam distillation.

Method: GC-MS was applied in this experiment.

Result: The ingredients and physical and chemical properties of essential oils of C.

[Analysis of the essential oils from Amomun kravak Pierre ex Gagnep by GC-MS].

Objective: To establish the fingerprint chromatogram of Amomun kravak Pierre ex Gagnep.

Methods: The composition of the essential oil was isolated by PR solvent and determined by GC-MS. Forty-two species of compounds were identified, of which the relative contents were calculated using square peaks to normalization, among which the character components were 1,8-cineole, beta-pinene, alpha-pinene, p-cymene, etc.

[Study on the supercritical carbon dioxide extraction of Brucea Javanica oil].

The technics of supercritical carbon dioxide extraction of Brucea Javanica for injection oil was studied. The effects of the conditions of extraction and separation on extraction oil yield and quality were investigated and the effects of extraction pressure and separation pressure on acid number of the oil were discussed. So the optimal condition of this method was determined.

[Determination of artemisinin in Artemisia annuna by HPLC-ELSD].

Objective: A HPLC method was first established to determine artemisinin in Artemisia annuna by HPLC-ELSD. Artemisinin in Herba of Artemisia annuna from different places was determined by this new method.

Methods: The method utilized MeOH-H2O (75: 25) as mobile phase with flow rate of 1 ml/min, C18 (250 x 4.

[Study on extraction of zhuyun 3# by supercritical CO2].

To optimize the extraction process for zhuyun 3# pill by supercritical CO2. Five kinds of supercritical CO2 extraction method had been applied. The methods were estimated by the pharmacological results and the content of flovone.