Morphological and molecular characteristics of swine coccidia based on single oocyst isolation.

Swine coccidiosis is a host-specific protozoan disease caused by Cystoisospora suis and various Eimeria species, leading to diarrhea or subclinical signs in pigs. In this study, 3296 fecal samples from 55 farms across six provinces in China were collected and examined to determine the prevalence and molecular characteristics of swine coccidia. The single oocyst isolation technique (SOIT) and molecular characterization identified nine coccidian species, with an overall infection prevalence of 13.

Wild sympatric rodents inhabiting pig farm environments may facilitate the spillover of Enterocytozoon bieneusi from pig farms.

Enterocytozoon bieneusi is a zoonotic pathogen prevalent in mammalian and avian hosts across the globe. Wild small mammals, being abundant worldwide, serve as important sources of zoonotic disease transmission to humans. Here, 227 fecal samples were collected from five rodent and shrew species on 34 pig farms in China to investigate the prevalence and molecular characterization of E.

Establishment and application of PDCoV antigen-specific DAS-ELISA detection method.

Background: Porcine deltacoronavirus (PDCoV) is a swine enteropathogenic coronavirus that affects young pigs, causing vomiting, acute diarrhea, dehydration, and even death. There is growing evidence that PDCoV can undergo cross-species as well as zoonotic transmissions. Due to the frequent outbreaks of this deadly virus, early detection is essential for effective prevention and control.

Predominance of the subtype ST5 among free-living sympatric rodents within pig farms in China suggests a novel transmission route from farms.

is a parasitic protist that can infect humans and various domestic and wild animals. However, there is limited research on the prevalence of this parasite among rodents, particularly those living in pig farm settings. Therefore, to investigate the occurrence, molecular characterization, and zoonotic potential of among rodents within pig farm environments, we conducted an investigation of 227 rodents and shrews from 34 pig farms located in Henan, Shaanxi, and Shanxi provinces of China using nested PCR of the SSU rRNA gene of .

Metagenomic analysis of gut microbiome and resistome of Whooper and Black Swans: a one health perspective.

Background: With the promotion of "One Health," the health of animals and their impact on the environment have become major concerns recently. Widely distributed in China, the whooper swans (Cygnus cygnus) and black swans (Cygnus atratus) are not only important to the ecological environment, but they may also potentially influence public health security. The metagenomic approach was adopted to uncover the impacts of the gut microbiota of swans on host and public health.

Screening putative antigens as stimulators in the Mycobacterium bovis interferon-gamma release assay for cattle.

Bovine tuberculosis (BTB) represents not only a significant economic concern, but also an important public health problem. Currently, interferon-gamma (IFN-γ) release assays (IGRAs) are widely used as an adjunct to the tuberculin test (TST) for the diagnosis of BTB. A great number of international studies have demonstrated that the sensitivity of the IFN-γ assay, which uses purified protein derivatives (PPDs) as diagnostic reagents, is superior to that of the TST.

[Development of an interferon-gamma ELISPOT for bovine tuberculosis].

We established an ELISPOT for bovine interferon-gamma (BoIFN-γ), and applied it in the diagnosis of bovine tuberculosis (bTB). Monoclonal antibodies that can bind with native BoIFN-γ were screened as the coating antibody and detecting antibody. After optimization of detecting conditions including coating antibody concentration, cell number, and detecting antibody concentration, the ELISPOT assay was established.

[Immunogenicity evaluation of Mycobacterium tuberculosis MPT83 protein and establishment of serological diagnostic method for bovine tuberculosis detection].

Objective: The aim of this study was to express Mycobacterium tuberculosis MPT83 protein and to evaluate its immunogenicity in murine model as well as the serological diagnosis potential value for bovine tuberculosis.

Methods: The fragment of mpt83 gene was amplified and constructed into pET30a(+)-mpt83 recombinant plasmid. MPT83 fusion protein was purified with His affinity chromatography column from strain of BL21(DE3)-pET30a(+)-mpt83 after induced by IPTG, and then used to evaluate its immunogenicity in mice and the potential application in ELISA assay for the detection of bovine tuberculosis.

[Prokaryotic expression and immunological characteristics of Mycobacterium tuberculosis Rv1886c].

Objective: Ag85B (Rv1886c) is secreted during the early stages of infection by Mycobacterium tuberculosis. The purpose of this study was probed into the immune response against Ag85B in vivo.

Methods: Ag85B was prokaryotic expressed and identified, its immunological characteristics were evaluated with indirect-ELSIA, Sandwich-ELISA and

Results: Ag85B was mainly expressed in form of inclusion body enzyme-linked immunospot assay (ELISPOT).

Generation and application of a 293 cell line stably expressing bovine interferon-gamma.

A stable mammalian cell line expressing highly active bovine interferon-gamma (BoIFN-γ) was generated using Flp recombinase-mediated integration. This recombinant 293 cell line (B1) efficiently secreted FLAG-tagged BoIFN-γ protein into the culture supernatant, as determined by ELISA and Western blot. The recombinant BoIFN-γ exhibited high anti-viral activity, suggesting that the 293 cells expressed BoIFN-γ that structurally and biologically resembled the natural protein.