Association Analysis of Single Nucleotide Polymorphisms in C1QTNF6, RAC2, and an Intergenic Region at 14q32.2 with Graves' Disease in Chinese Han Population.

Background: Variation within the C1QTNF6 gene at 22q12.3, the RAC2 gene at 22q13.1, and an intergenic region at 14q32.

Association of 4p14 and 6q27 variation with Graves disease: a case-control study and a meta-analysis of available evidence.

Background: The etiology of the Graves' disease (GD) is largely unknown. However, genetic factors are believed to play a major role. A recent genome-wide association study in a Han Chinese sample collection revealed two new Graves' disease (GD) risk loci within chromosome band 4p14 and 6q27.

[Effect of phenformin hydrochloride on pharmacokinetics of puerarin in rats].

Objective: To study the effect of phenformin hydrochloride that may be illegally added in traditional Chinese medicine preparations on the pharmacokinetics of puerarin in rats.

Method: Rats were randomly divided into the single pueraria group and the phenformin hydrochloride combined with pueraria group. After oral administration in the two groups, their bloods were sampled at different time points to determine the drug concentration of puerarin in rat blood and calculate pharmacokinetic parameters.

[LC-MS/MS method for quantification and pharmacokinetic study of gabapentin in human plasma].

A sensitive, rapid and specific liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for quantification of gabapentin in human plasma has been developed. After a single plasma protein precipitation with methanol, gabapentin and metformin (internal standard) were chromatographed on a Inertsil ODS-3 column (50 mm x 2.1 mm ID, 3 microm) with mobile phase consisting of methanol-0.

Cytotoxic constituents from the skin of the toad Bufo bufo gargarizans.

To study the chemical composition of the skin of Bufo bufo gargarizans, various chromatographic methods were used in the isolation procedures and the structures of isolated compounds were determined based on NMR and MS analysis. As a result, two new compounds were isolated from its ethanolic extract and characterized as N-[2-hydroxy-2-(4-hydroxyphenyl)ethyl]-N-methylurea (1) and 19-oxocinobufotalin 3-adipoylarginine ester (2), together with 11 known compounds. Isolated bufadienolides showed significant inhibition effect against human hepatocellular carcinoma cell line SMMC-7721 in vitro.

[Studies on chemical constituents of the extract of Lonicera japonica].

Objective: To isolate and elucidate the structure of the constituents of the extract of Lonicera japonica.

Methods: The compounds were isolated and repeatedly purified on TLC, silica gel column chromatography, and gel column chromatography. The structures were elucidated by physico-chemical properties, MS and NMR.

Investigation on the chemical constituents and variation of the flower buds of Lonicera species by UPLC-ESI-MS/MS and principle component analysis.

A rapid ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric (UPLC-ESI-MS/MS) method is developed for the qualitative identification of constituents in the flower buds of seven Lonicera species. The optimal condition of separation and detection were achieved on an AcQuity UPLC BEH C18 column with a gradient elution with acetonitrile and 0.1% acetic acid within 17 min.

[Determination of mosapride in human plasma by high performance liquid chromatography tandem mass spectrometry].

To develop a sensitive and specific high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method for the determination of mosapride in human plasma, mosapride and internal standard tamsulosin were extracted from plasma with liquid-liquid extraction, then separated on a Waters ACQUITY UPLC BEH C18 column (50 mm x 2.1 mm, 1.7 microm ID) with gradient elution at flow-rate of 0.

[Fingerprint analysis of composite Folii Isatidis injection by HPLC].

Objective: To develop the chromatographic fingerprints of composite Folii Isatidis injection by HPLC.

Method: The separation was performed on a Diamonsil C18 column with a mobile phase consisting of methanol-water-phosphoric acid as gradient eluent at the flow rate of 1.0 mL x min(-1).

Enantiomeric separation of fluoxetine derivatives on polysaccharide-based chiral columns.

A high performance liquid chromatography (HPLC) method employing amylose-based chiral columns (Chiralpak AD-RH and Chiralpak AD) and cellulose-based chiral columns (Chiralcel OD) as chiral stationary phases have been developed for the enantiomeric separation of fluoxetine (FLX) derivatives. The FLX was derivatized with 4-(N-chloroformylmethyl-N-methyl)amino-7-nitro-2,1,3-benzoxadiazole (NBD-COCl) and 4-(N-chloroformylmethyl-N-methyl)amino-7-N,N-dimethylaminosulfonyl-2,1,3-benzoxadiazole (DBD-COCl), respectively. Influence of the mobile phase composition and column temperature on the enantioseparation was discussed during the separation.

[Chemical constituents from herb of Epimedium brevicornum].

Objective: To investigate the chemical constituents of Epimedium brevicornum.

Method: The chemical constituents were isolated by using silica gel column chromatography and preparative TLC. The structures were identified on the basis of physical-chemical constants and spectral data.

[Liquid chromatography frontal analysis of the protein binding of glimepiride].

Aim: To study the protein binding of glimepiride.

Methods: An HPLC-FA method is performed by using Pinkerton GFF II-S5-80 internal-surface reversed-phase silica support (150 mm x 4.6 mm ID, 5 microm) at pH 7.

[Preparation and simultaneous determination of corynoline and acetylcorynoline in the herb of Corydalis bungeana].

Objective: To isolate and purify corynoline and acetylcorynoline from Corydalis bungeana and develop a reversed-phase HPLC method of determining the two components in C. bungeana.

Method: Alkaloids were isolated from the ethanolic extract with column gel chromatography, and identified on the basis of spectral analysis (UV, 1H-NMR, 13C-NMR) and physicochemical properties.

[Determination of adenosine, rutin and quercetin in Carthamus tinctorius by HPCE].

Aim: To develop a method for determination of adenosine, rutin and quercetin in Carthamus tinctorius L. by high performance capillary electrophoresis(HPCE).

Methods: A fused silica capillary (66.

[HPLC determination of two flavonoid compounds in Psoralea corylifolia].

Objective: To determine two flavonoid compounds in Psoralea corylifolia L. (PC) simultaneously with HPLC method.

Method: Bavachin and corylin isolated from PC and purified in our laboratory were used as the reference compounds.

[Determination of trigonelline in Trigonella foenum-graecum by HPLC].

Objective: A HPLC method is established to determine the content of trigonelline in Trigonella foenum-graecum.

Method: The medicinal material was extracted by petholeum ether-ethanol. Asahipak NH2P-50 column was used, mobilephase consisted of acetonitrile-water(75:25) and detection wavelength was set at UV 265 nm.