Studies on the immune response to a characterized antigenic determinant of the tobacco mosaic virus protein.

THE FOLLOWING PEPTIDES HAVE PREVIOUSLY BEEN SHOWN TO BIND SPECIFICALLY WITH ANTIBODIES TO TMVP: (a) An eicosapeptide representing residues 93-112 of TMVP and having the sequence Ileu-Ileu-Glu-Val-Glu-AspNH(2)-GluNH(2)-Ala-AspNH(2)-Pro-Thr-Thr-Ala-Glu-Thr-Leu-Asp-Ala-Thr-Arg. (b) Its C-terminal decapeptide. (c) Its C-terminal pentapeptide.

The hyperviscosity syndrome. I. In IgG myeloma. The role of protein concentration and molecular shape.

The hyperviscosity syndrome is an uncommon complication in IgG myeloma. Its occurrence has been ascribed to the presence in the serum of high molecular weight polymers of the IgG proteins. Three patients with IgG myeloma and the clinical hyperviscosity syndrome were investigated, none of whom had IgG polymers in the serum by analytical ultracentrifugation.

The effect of anti-lymphocyte globulin on human monocytes in vitro.

Purified human monocytes were incubated with horse anti-human-lymphocyte globulin (ALG) and the effect of this treatment on some of their functions was evaluated. Cytotoxic antibodies against monocytes and inhibition of phagocytosis of polystyrene particles were detected in the presence of high concentrations of ALG. By contrast, ALG interfered effectively with the γG-receptor on monocytes and this inhibition was detectable even at high dilutions of the antisera.

Am(1), the first genetic marker of human immunoglobulin A.

The genetic markers of human immunoglobulins have significantly contributed to the understanding of the molecular biology of antibody synthesis. Like the Gm markers of IgG, the first genetic marker of serum IgA, a major immunoglubulin of exocrine secretions, has now been defined and termed Am(1). It is inherited as a Mendelian dominant trait and is independent of the Gm and Inv allotypes.

In vivo metabolism of complement. I. Metabolism of the third component (C'3) in acquired hemolytic anemia.

The in vivo metabolism of purified third component of complement labeled with (125)-iodine (C'3-(125)I) was studied in normal subjects and in patients with acquired hemolytic anemias. 27 such studies were performed; in addition, three studies were performed using C'3i, the biologically inactive reaction product of C'3. In normal subjects the mean fractional catabolic rate of C'3 was 2.

The IgG receptor: an immunological marker for the characterization of mononuclear cells.

Using red cells sensitized with an IgG anti-Rh antibody, an IgG receptor was demonstrable on human monocytes, hepatic macrophage and splenic macrophage preparations. The receptor was uniformly lacking on lymphocytes, lymphoid cell lines and lymphocytes stimulated with phytomitogens . The integrity of this receptor was demonstrated on monocytes obtained from patients with `acquired' agammaglobulinaemia, chronic granulomatous disease and acute monocytic leukaemia.