HIV Modulates Osteoblast Differentiation via Upregulation of RANKL and Vitronectin.

Bone loss is a prevalent characteristic among people with HIV (PWH). We focused on mesenchymal stem cells (MSCs) and osteoblasts, examining their susceptibility to different HIV strains (R5- and X4-tropic) and the subsequent effects on bone tissue homeostasis. Our findings suggest that MSCs and osteoblasts are susceptible to R5- and X4-tropic HIV but do not support productive HIV replication.

Bystander Effects and Profibrotic Interactions in Hepatic Stellate Cells during HIV and HCV Coinfection.

This study aims to explore the influence of coinfection with HCV and HIV on hepatic fibrosis. A coculture system was set up to actively replicate both viruses, incorporating CD4 T lymphocytes (Jurkat), hepatic stellate cells (LX-2), and hepatocytes (Huh7.5).

HIV-Infected Hepatic Stellate Cells or HCV-Infected Hepatocytes Are Unable to Promote Latency Reversal among HIV-Infected Mononuclear Cells.

Due to a common mode of transmission through infected human blood, hepatitis C virus (HCV) and human immunodeficiency virus (HIV) co-infection is relatively prevalent. In alignment with this, HCV co-infection is associated with an increased size of the HIV reservoir in highly active antiretroviral therapy (HAART)-treated individuals. Hence, it is crucial to comprehend the physiological mechanisms governing the latency and reactivation of HIV in reservoirs.

Viable SARS-CoV-2 Omicron sub-variants isolated from autopsy tissues.

Introduction: Pulmonary and extrapulmonary manifestations have been described after infection with SARS-CoV-2, the causative agent of coronavirus disease 2019 (COVID-19). The virus is known to persist in multiple organs due to its tropism for several tissues. However, previous reports were unable to provide definitive information about whether the virus is viable and transmissible.

Infection Promotes an Imbalance in the Adipocyte-Osteoblast Crosstalk Favoring Bone Resorption.

Osteoarticular injury is the most common presentation of active brucellosis in humans. Osteoblasts and adipocytes originate from mesenchymal stem cells (MSC). Since those osteoblasts are bone-forming cells, the predilection of MSC to differentiate into adipocytes or osteoblasts is a potential factor involved in bone loss.

Herpes simplex virus alters Alzheimer's disease biomarkers - A hypothesis paper.

Introduction: Human herpes simplex virus 1 (HSV1) is discussed to induce amyloid-β (Aβ) accumulation and neurofibrillary tangles of hyperphosphorylated tau (pTau) in Alzheimer's disease (AD) in cell culture and animal models. Aβ appears to be virostatic. We investigated the association between intrathecal antibodies against HSV or cytomegalovirus (CMV) and cerebrospinal fluid (CSF) AD biomarkers.

Proinflammatory Microenvironment During Infection Modulates Osteoclastogenesis.

is an emerging pathogen that causes septic arthritis, osteomyelitis, and bacteremia in children from 6 to 48 months of age. The presence of bacteria within or near the bone is associated with an inflammatory process that results in osteolysis, but the underlying pathogenic mechanisms involved are largely unknown. To determine the link between and bone loss, we have assessed whether infection or through the genesis of a pro-inflammatory microenvironment can promote osteoclastogenesis.

Calcific tendinitis in the proximal thigh.

Calcific tendinitis of the proximal thigh is a benign entity that may cause significant pain. It must be distinguished from chronic or malignant disorders, such as arthritis, infection, and soft tissue/cortical neoplasms. Although it may be self-limited, some patients will benefit from medical intervention.

Synovial chondromatosis of the hip. Evaluation with air computed arthrotomography.

Four patients with primary synovial chondromatosis of the hip were evaluated with air or double contrast computed arthrotomography. Air computed arthrotomography was an easy and accurate method of diagnosing the disease and delineating its anatomic extent. The use of air as a sole contrast agent allowed noncalcified chondromatous nodules to be identified.

Thoughts on the diagnosis of bone tumors.

The pathologist and the radiologist have each been given reason to believe that he is capable of accurately diagnosing bone tumors without the assistance of the other. However, each specialist has recourse to tools and procedures not available to the other, and their findings should be considered as complementary. Although there are radiographic features which could be an indication of malignancy, there are too many common exceptions to formulate a general rule.

Tarsal navicular stress fractures: radiographic evaluation.

Tarsal navicular stress fractures are a potential source of disabling foot pain in physically active individuals. The diagnosis of tarsal navicular stress fracture requires a high index of clinical and radiographic suspicion because the fracture is only rarely evident on routine radiographs or standard tomograms. The radiographic diagnosis of a tarsal navicular stress fracture may require anatomic anteroposterior tomograms or a radionuclide bone scan with plantar views.

Percutaneous bone biopsy: the importance of aspirated osseous blood.

The diagnostic value of the microscopic examination of bone core specimen versus osseous blood, obtained by 110 percutaneous biopsies performed on 100 patients, was evaluated. A diagnosis of malignancy was made by biopsy in 54 cases. In 52 cases in which osseous blood (clot and smears) was available for examination, a positive diagnosis for malignancy was made microscopically in 49 (94%).

Closed skeletal needle biopsy: review of 120 cases.

A review of 120 percutaneous bone biopsies performed on 110 patients in the Radiology Department of the Hospital for Special Surgery showed a 72% concurrence of biopsy findings with the patient's other clinical findings and subsequent course. Of the biopsies, 58% were correctly positive for malignancy or infection and 14% were correctly negative. The rest, 28% of the biopsies, were either incorrectly negative or had insufficient tissue for diagnosis and were therefore unsatisfactory.

Radiologic evaluation of painful joint prostheses.

The most important method of evaluation of painful total joint prostheses is review of sequential radiographs. Because clinical failure from loosening occurs late, abnormalities of alignment, the cement-bone or cement-metal interface, the bony contour, or the integrity of the prosthetic components will be found in the vast majority of cases of clinical failure. In cases in which plain radiographs are normal or equivocal, radionuclide bone scanning with Tc-99m diphosphonate agents are useful.

Untreated congenital hip dysplasia in the Navajo.

Congenital hip dysplasia without dislocation was identified in 18 Navajo Indian children. Treatment had been declined and the children presented a unique opportunity to observe the natural course of hip dysplasia. In a follow-up period of three to 19 years (average, 11.

Radiological evaluation of painful total hip replacement.

Ninety-four cases of clinically failed, cemented, total hip prostheses requiring surgery were reviewed to determine the accuracy of preoperative plain radiography, culture of aspirated fluid, arthrography, and bone scanning. When radiopaque cement had been used to embed the prosthesis, plain radiography was highly accurate in detecting a loose femoral component, less so in detecting a loose acetabular component. Culture of aspirated fluid was accurate in diagnosing infection.

Infantile coxa vara.

Infantile coxa vara is an unusual localized dysplasia, not evident at birth, which is first noticed when the child begins walking. Twelve cases are presented with emphasis on the characteristic roentgen findings which include abnormal ossification of the femoral neck, a vertical physis, a characteristic triangular osseous fragment at the medial inferior corner of the metaphysis, and a straight femoral shaft. Prompt diagnosis and early management can reduce severe deformity and degenerative changes of the hip.