Distribution of Taenia saginata cysticerci in tissues of experimentally infected cattle.

Bovine cysticercosis caused by Taenia saginata is a zoonotic disease warranting routine inspection measures for the postmortem detection of cysticerci (cysts) in beef destined for human consumption. Detection is based on gross examination of traditional carcass predilection sites, although there is evidence to suggest that examination of other sites may offer improvements in sensitivity. In order to evaluate the efficacy of current inspection protocols, this study determined the distribution and number of cysticerci in the tissues of experimentally infected cattle.

Chronic disease-related lifestyle risk factors in a sample of Canadian adolescents.

This study examined the prevalence, clustering, age trends, and gender differences of chronic-disease related risk factors among a large sample of adolescents (N = 4932) in Alberta, Canada. Approximately 43% of boys and 53% of girls displayed two or more risk factors. Age trends and gender differences were also observed.

Chronic exposure to outdoor air pollution and lung function in adults.

Background: The extent to which chronic exposure to outdoor air pollutants influences lung function in adults is unclear. The aim of this study was to measure the association between chronic exposure to outdoor air pollutants and adult lung function.

Methods: The relationship between measures of lung function (forced expiratory volume in 1 s (FEV(1)) and FEV(1) as a percentage of forced vital capacity (FVC)) and average exposure to particulate matter <10 microm in diameter, nitrogen dioxide, sulfur dioxide and ozone was examined in four representative cross-sectional surveys of the English population aged > or =16 in 1995, 1996, 1997 and 2001.

Chronic exposure to outdoor air pollution and markers of systemic inflammation.

Background: Cohort studies suggest that long-term exposure to higher levels of outdoor air pollution increases risk of developing cardiovascular disease. One suggested mechanism is that air pollution, especially particulate matter, induces systemic inflammation, thereby increasing the risk of developing long-term pathologic changes in the cardiovascular system, We aimed to examine the association between long-term exposure to higher levels of air pollution and chronic systemic inflammation.

Methods: We examined the relationship between 2 markers of systemic inflammation (fibrinogen and C-reactive protein) and measures of outdoor air pollution estimated for each postcode sector of residence, using models incorporating information on pollutant emissions from multiple sources, and atmospheric dispersion and processing, in 3 representative cross-sectional studies of the English population in 1994, 1998, and 2003.

3D Gradient coil design - toroidal surfaces.

Gradient coil design typically involves optimisation of current densities or coil windings on familiar cylindrical, planar, spherical or conical surfaces. In this paper, an analytic inverse method is presented for the theoretical design of toroidal transverse gradient coils. This novel geometry is based on previous work involving a 3D current density solution, in which the precise geometry of the gradient coils was obtained as part of the optimisation process.

3-D gradient coil design--initial theoretical framework.

An analytic inverse method is presented for the theoretical design of 3-D transverse gradient coils. Existing gradient coil design methods require the basic geometry of the coil to be predetermined before optimization. Typically, coil windings are constrained to lie on cylindrical, planar, spherical, or conical surfaces.

Trichinella diagnostics and control: mandatory and best practices for ensuring food safety.

Because of its role in human disease, there are increasing global requirements for reliable diagnostic and control methods for Trichinella in food animals to ensure meat safety and to facilitate trade. Consequently, there is a need for standardization of methods, programs, and best practices used in the control of Trichinella and trichinellosis. This review article describes the biology and epidemiology of Trichinella, and describes recommended test methods as well as modified and optimized procedures that are used in meat inspection programs.

Polyhexamethylene guanidine hydrochloride-based disinfectant: a novel tool to fight meticillin-resistant Staphylococcus aureus and nosocomial infections.

Polyhexamethylene guanidine hydrochloride (PHMGH), an antimicrobial biocide of the guanidine family, was tested for efficacy against quality-control strains of Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella choleraesuis, meticillin-resistant S. aureus (MRSA) and Escherichia coli. Bactericidal activity against S.

Canine brucellosis in a Saskatchewan kennel.

Canine brucellosis is rare in Canada. This report describes an outbreak of Brucella canis infection within a kennel, emphasizing diagnostic and pathologic findings. Gender differences are described.

Complete validation of a unique digestion assay to detect Trichinella larvae in horse meat demonstrates the reliability of this assay for meeting food safety and trade requirements.

A tissue digestion assay using a double separatory funnel procedure for the detection of Trichinella larvae in horse meat was validated for application in food safety programs and trade. The assay consisted of a pepsin-HCl digestion step to release larvae from muscle tissue and two sequential sedimentation steps in separatory funnels to recover and concentrate larvae for detection with a stereomicroscope. With defined critical control points, the assay was conducted within a quality assurance system compliant with International Organization for Standardization-International Electrotechnical Commission (ISO/IEC) 17025 guidelines.

Both maternal sensitivity and atypical maternal behavior independently predict attachment security and disorganization in adolescent mother-infant relationships.

Extant theory holds that variation in attachment security is largely determined by caregiver sensitivity whereas disorganization has its roots in atypical interactions that frighten the infant. These hypotheses were confirmed in the current study of a high-risk sample but, contrary to current theory, both atypical maternal behavior and maternal sensitivity were also significant independent predictors of attachment disorganization and security.

Use of proficiency samples to assess diagnostic laboratories in France performing a Trichinella digestion assay.

Routine diagnosis of animal trichinellosis for food safety and trade relies on a method of artificial digestion to free Trichinella muscle larvae from meat for subsequent identification by microscopy. As part of a quality control system, the French National Reference Laboratory (NRL) initiated ring trials to determine the sensitivity of the test performed in the 72 routine diagnostic laboratories in France. A method was devised to obtain calibrated meat samples containing known numbers of capsules with Trichinella spiralis muscle larvae.

Similarities and differences in mothers' and observers' ratings of infant security on the Attachment Q-Sort.

This study examined the similarities and differences in maternal and observer Attachment Behavior Q-Sort ratings (AQS; Waters, 1995) and their relations to dimensions of the developmental ecology - maternal sensitivity, infant irritability, parental stress and psychosocial risk. Data was gathered from low risk (adult mothers; N=44) and high risk mother-infant dyads (adolescent mothers; N=83) when infants were aged 6, 10 and 15 months old, attachment being assessed at 15 months by both mothers and independent observers. A common factor was extracted from both sorts and served to operationalize the similarities between mother and observer ratings.

Change in atypical maternal behavior predicts change in attachment disorganization from 12 to 24 months in a high-risk sample.

This longitudinal study examined links between disorganization and atypical maternal behavior at 12 and 24 months in 71 adolescent mother-child dyads. Organized attachment and maternal not disrupted behavior were more stable than disorganization and disrupted behavior, respectively. At both ages, disorganization and maternal disrupted behavior were significantly correlated.

Risk factors for accident and emergency (A&E) attendance for asthma in inner city children.

Background: Inner city children make heavy use of accident and emergency (A&E) services for asthma. Developing strategies to reduce this requires a better understanding of the risk factors.

Methods: A case-control study was carried out of children with asthma living in south-east London: 1018 children who attended A&E for asthma over 1 year and 394 children who had not attended A&E for asthma over the previous year.

Viability and infectivity of Trichinella spiralis muscle larvae in frozen horse tissue.

Many aspects of the biology and epidemiology of Trichinella infection in the horse are poorly understood, including survival of Trichinella spp in horse muscle. In this study, we have assessed the freeze tolerance of T. spiralis in horse meat stored at 5, -5, and -18 degrees C for 1 day to 24 weeks.

Larval viability and serological response in horses with long-term Trichinella spiralis infection.

The horse is considered an aberrant host for the nematode parasite Trichinella spiralis, and many aspects of the biology and epidemiology of Trichinella infection in the horse are poorly understood. It has been reported that experimentally-infected horses produce a transient serological response to infection and that muscle larvae are cleared more rapidly than in parasite-adapted hosts such as the pig and humans. However, limited numbers of animals have been studied, and both the longevity of larvae in horse musculature and the immune response to Trichinella larvae remain unclear.

Overview of food- and water-borne zoonotic parasites at the farm level.

Zoonotic parasites found in food animals include a wide variety of protozoa, nematodes, trematodes, and cestodes. Many of these parasites are emerging or already occur globally due to changes in farming practices and the increased movement of animals, food, and people. Some of the emerging or ubiquitous parasites, including Toxoplasma, Cryptosporidium, Trichinella, and Taenia, present enormous risks to global food production and consumer health.

Towards single screening tests for brucellosis.

This paper describes an indirect enzyme-linked immunosorbent assay (I-ELISA) and a fluorescence polarisation assay (FPA), each capable of detecting antibody in several species of hosts to smooth and rough members of the genus Brucella. The I-ELISA uses a mixture of smooth lipopolysaccharide (SLPS) and rough lipopolysaccharide (RLPS) as the antigen, and a recombinant protein A/G conjugated with horseradish peroxidase as the detection reagent. When using individually determined cutoff values, the SLPS/RLPS combined-antigen I-ELISA detected antibody in slightly more animals exposed to SLPS or to RLPS than did I-ELISA procedures using each individual antigen separately.

The finished DNA sequence of human chromosome 12.

Human chromosome 12 contains more than 1,400 coding genes and 487 loci that have been directly implicated in human disease. The q arm of chromosome 12 contains one of the largest blocks of linkage disequilibrium found in the human genome. Here we present the finished sequence of human chromosome 12, which has been finished to high quality and spans approximately 132 megabases, representing approximately 4.

A program to accredit laboratories for reliable testing of pork and horse meat for Trichinella.

The Canadian Food Inspection Agency (CFIA) has developed a program to accredit external laboratories to conduct Trichinella digestion assays for export purposes. Accredited laboratories are responsible for staffing, equipment and operating test facilities under the auspices and guidance of the CFIA. The CFIA's Centre for Animal Parasitology provides training, proficiency samples, audits and other support for the accreditation process.

Industry role in education--meeting the demand.

A relative decline in the use of peritoneal dialysis (PD) as a treatment modality has led to much speculation regarding the reasons for the drop. One main concern focuses on the lack of education and training in dialysis options for nephrologists, nurses, and patients. To change the trend and improve clinical outcomes for dialysis patients, the industry is taking a leading role in educating health care professionals and patients about dialysis options.

Comparison of synthetic tyvelose antigen with excretory-secretory antigen for the detection of trichinellosis in swine using enzyme-linked immunosorbent assay.

Two enzyme-linked immunosorbent assay (ELISA) systems, one using natural excretory-secretory (ES) antigens and the other a synthetic glycan antigen (3,6-dideoxy-D-arabinohexose [tyvelose, TY]), were evaluated for the serological diagnosis of trichinellosis in swine. Sensitivity was estimated using samples (n = 113) collected 3-21 wk PI from 15 experimentally infected pigs, and specificity was estimated using samples (n = 397) from a population of Trichinella spp.-free pigs.