Statistical analysis plan for hemodynamic phenotype-based, capillary refill time-targeted resuscitation in early septic shock: the ANDROMEDA-SHOCK-2 randomized clinical trial.

Background: ANDROMEDA-SHOCK 2 is an international, multicenter, randomized controlled trial comparing hemodynamic phenotype-based, capillary refill time-targeted resuscitation in early septic shock to standard care resuscitation to test the hypothesis that the former is associated with lower morbidity and mortality in terms of hierarchal analysis of outcomes.

Objective: To report the statistical plan for the ANDROMEDA--SHOCK 2 randomized clinical trial.

Methods: We briefly describe the trial design, patients, methods of randomization, interventions, outcomes, and sample size.

Treatment of pediatric drug-resistant generalized epilepsy with responsive neurostimulation of the centromedian nucleus of the thalamus: A case series of seven patients.

Purpose: Responsive neurostimulation of the centromedian nucleus of the thalamus (CM RNS) is being investigated for treatment of drug-resistant generalized epilepsy with promising results. The aim of this study is to report outcomes of seven patients with pediatric-onset drug-resistant generalized epilepsy, including both genetic generalized epilepsy (GGE) and Lennox-Gastaut syndrome (LGS), who underwent treatment with bilateral CM RNS.

Methods: A retrospective chart review was performed for patients with drug-resistant generalized epilepsy who underwent treatment with bilateral CM RNS at Children's Hospital of Pittsburgh from 2020 to 2022.

Multipronged impact of environmental temperature on Staphylococcus aureus infection by phage Kayvirus rodi: Implications for biofilm control.

Environmental cues sometimes have a direct impact on phage particle stability, as well as bacterial physiology and metabolism, having a profound effect on phage infection outcome. Here, we explore the impact of temperature on the interplay between phage (phiIPLA-RODI) and its host, . Our results show that phiIPLA-RODI is a more effective predator at room (25 °C) compared to body temperature (37 °C) against planktonic cultures of several strains with varying degrees of phage susceptibility.

Liver Xenotransplantation: A Path to Clinical Reality.

Liver xenotransplantation has emerged as a potential solution to the shortage of deceased human donor organs and is now becoming a reality due to recent developments in genetic engineering and immunosuppressive therapy. Early efforts using non-human primates and genetically modified pigs faced significant challenges such as thrombocytopenia and graft rejection. Understanding the mechanism behind those challenges and using novel genetically engineered pigs enabled researchers to overcome some of the hurdles, but more research is needed.

Younger adults may be faster at making semantic predictions, but older adults are more efficient.

While there is strong evidence that younger adults use contextual information to generate semantic predictions, findings from older adults are less clear. Age affects cognition in a variety of different ways that may impact prediction mechanisms; while the efficiency of memory systems and processing speed decrease, life experience leads to complementary increases in vocabulary size, real-world knowledge, and even inhibitory control. Using the visual world paradigm, we tested prediction in younger ( = 30, between 18 and 35 years of age) and older adults ( = 30, between 53 and 78 years of age).

Effectiveness of Body Remodeling and Cellulite Appearance Improvement Treatments in the Thighs Using Symmed Radiofrequency Device.

Background: Aging associated with the passage of time causes alterations in subcutaneous tissues and the overall appearance of the skin that generate aesthetic unconformities in people. Within the wide range of available techniques on the market, radiofrequency (RF) diathermy has emerged as one of the most used non-invasive methods to combat them.

Objective: This clinical trial aims to determine and quantify the effectiveness and safety of the high-frequency RF-based device Symmed (Termosalud Inc.

Characterizing astrocyte-mediated neurovascular coupling by combining optogenetics and biophysical modeling.

Vasoactive signaling from astrocytes is an important contributor to the neurovascular coupling (NVC), which aims at providing energy to neurons during brain activation by increasing blood perfusion in the surrounding vasculature. Pharmacological manipulations have been previously combined with experimental techniques (e.g.

The Safety of Probiotics Intended for Use in Pregnant and Lactating Women: From a Desirable to a Required Task.

During pregnancy, women undergo changes that affect virtually every organ, apparatus, or system, including the host microbiota. Most pregnancies progress smoothly despite the common presence of minor side-effects arising from such adaptations. However, some women may experience more serious complications, including gestational diabetes mellitus, preeclampsia, or preterm delivery.

Telomere-based risk models for the early diagnosis of lung cancer.

Background: The objective of this study was to evaluate the use of telomere length measurements as diagnostic biomarkers during early screening for lung cancer in high-risk patients.

Methods: This was a prospective study of patients undergoing lung cancer diagnosis at two Spanish hospitals between April 2017 and January 2020. Telomeres from peripheral blood lymphocytes were analysed by Telomere Analysis Technology, which is based in high-throughput quantitative fluorescent in situ hybridization.

Key steps in exposure techniques for robotic total mesorectal excision (TME).

Aim: The use of robotic surgery is increasing significantly. Specific training is fundamental to achieve high quality and better oncological outcomes. This work defines key exposure techniques in robotic total mesorectal excision (TME).

Developing a Bilingual Health Education Program to Identify and Address Healthcare Misinformation in a Border Community.

El Paso Health Education and Awareness Team (EP-HEAT®) is a bilingual program focused on increasing health awareness and dispelling health-related misinformation in the U.S.-Mexico border region.

Assessment of total mercury content in fish muscle tissue from the middle basin of the Pastaza River, Ecuador.

One of the most toxic metals is mercury, which exhibits high toxicity during short exposure periods. This study aimed to determine the concentration of total mercury (THg) in fish muscle tissue from various species captured from two locations in the middle basin of the Pastaza River in Ecuador, which the locals use in their weekly diet. The individuals captured belong to the following families: Loricariidae, Cetopsidae, Heptapteridae, Cichlidae, Parodontidae, Characidae, Prochilodontidae, Curimatida, Siluriformes, Cichliformes, Characiformes, Erythrinidae and Curimatidae.

Evaluation of a Non-Enzymatic Electrochemical Sensor Based on Co(OH)-Functionalized Carbon Nanotubes for Glucose Detection.

This work reports on the assessment of a non-hydrolytic electrochemical sensor for glucose sensing that is developed using functionalized carbon nanotubes (fCNTs)/Co(OH). The morphology of the nanocomposite was investigated by scanning electron microscopy, which revealed that the CNTs interacted with Co(OH). This content formed a nanocomposite that improved the electrochemical characterizations of the electrode, including the electrochemical active surface area and capacitance, thus improving sensitivity to glucose.

Rome Foundation and international neurogastroenterology and motility societies' consensus on idiopathic gastroparesis.

To establish a consensus on the definition and management of idiopathic gastroparesis, international experts (selected by neurogastroenterology and motility societies and initiated by the Rome Foundation) devised 144 statements using the Delphi method, with at least 80% agreement required. This consensus defined idiopathic gastroparesis as the presence of symptoms associated with delayed gastric emptying in the absence of mechanical obstruction. Nausea and vomiting were identified as cardinal symptoms.

Deciphering the lung microbiota in COVID-19 patients: insights from culture analysis, FilmArray pneumonia panel, ventilation impact, and mortality trends.

Few studies have analyzed the role of the lung microbiome in the diagnosis of pulmonary coinfection in ventilated ICU COVID-19 patients. We characterized the lung microbiota in COVID-19 patients with severe pneumonia on invasive mechanical ventilation using full-length 16S rRNA gene sequencing and established its relationship with coinfections, mortality, and the need for mechanical ventilation for more than 7 days. This study included 67 COVID-19 ICU patients.

Independent effects of long and short-term exposures to non-optimal increased temperature on mortality.

Although the short-term heat effects are well-established, longer-term effects, beyond those, have recently received attention, in the context of climate change. Our study aims to investigate the potential effects of long-term exposure to non-optimal warm period temperatures on all-cause mortality in four large regions in the UK, Norway, Italy, and Greece. Daily all-cause mortality counts from 1996 to 2018 for four European NUTS-2 regions including 52-662 small areas were collected and associated with spatiotemporal temperature estimates.

A patient reported outcome measure for rectal cancer patients eligible for organ preservation: Development and validation of a Watch-and-Wait module for the Assessment of Burden of disease in ColoRectal Cancer (ABCRC) tool.

Aim: The Assessment of Burden of ColoRectal Cancer (ABCRC)-tool is an integrated tool, developed in conjunction with colorectal cancer (CRC) patients, that measures the experienced burden of disease and lifestyle parameters and visualizes the results. To provide tailored follow-up care for watch-and-wait (WW) patients, in line with their specific needs and preferences, a WW module for the ABCRC-tool was developed. In this paper we describe the development and validation process of the WW module.

Evaluation of a Method for the Quantification of Cadmium, Lead, and Zinc in Craft Beers Manufactured in Quito, Ecuador.

The brewing process of craft beer can introduce contamination by heavy metals such as Cd, Pb, and Zn from various sources. Cadmium and lead metals are particularly worrisome because of their harmful effects on human health. This study aimed to evaluate a method for quantifying the levels of Cd, Pb, and Zn in craft beer brands sold in the northern region of the Metropolitan District of Quito, Ecuador, using atomic absorption spectroscopy.

Infraslow noradrenergic locus coeruleus activity fluctuations are gatekeepers of the NREM-REM sleep cycle.

The noradrenergic locus coeruleus (LC) regulates arousal levels during wakefulness, but its role in sleep remains unclear. Here, we show in mice that fluctuating LC neuronal activity partitions non-rapid-eye-movement sleep (NREMS) into two brain-autonomic states that govern the NREMS-REMS cycle over ~50-s periods; high LC activity induces a subcortical-autonomic arousal state that facilitates cortical microarousals, whereas low LC activity is required for NREMS-to-REMS transitions. This functional alternation regulates the duration of the NREMS-REMS cycle by setting permissive windows for REMS entries during undisturbed sleep while limiting these entries to maximally one per ~50-s period during REMS restriction.