C-Peptide, Baseline and Postprandial Insulin Resistance after a Carbohydrate-Rich Test Meal - Evidence for an Increased Insulin Clearance in PCOS Patients?

Known characteristics of patients with PCOS include infertility, menstrual disorders, hirsutism and also often insulin resistance. These symptoms increase with increasing body weight. In the LIPCOS study (ifestyle ntervention for Patients with Polycystic Ovary Syndrome []) long-term changes of the PCOS in dependence on pregnancy and parenthood were systematically assessed.

Ca2+-activated, large conductance K+ channel in the ovary: identification, characterization, and functional involvement in steroidogenesis.

Progesterone production by the corpus luteum is a process vital for reproduction. In humans its secretion is stimulated by the placental hormone human chorionic gonadotropin (hCG), and this stimulatory action can also be observed in cultured human luteinized granulosa cells (GCs). We now provide evidence that opening of a Ca(2+)-activated K(+) channel, the BK(Ca), is crucially involved in this process.

Identification of an ovarian voltage-activated Na+-channel type: hints to involvement in luteolysis.

An endocrine type of voltage-activated sodium channel (eNaCh) was identified in the human ovary and human luteinized granulosa cells (GC). Whole-cell patch-clamp studies showed that the eNaCh in GC is functional and tetrodotoxin (TTX) sensitive. The luteotrophic hormone human CG (hCG) was found to decrease the peak amplitude of the sodium current within seconds.

Presence of transforming growth factor alpha and epidermal growth factor in human ovarian tissue and follicular fluid.

Intra-ovarian regulation of follicular maturation is modulated by various factors. Among these, growth factors are important local actors. We examined the immunohistochemical localization of transforming growth factor alpha (TGF-alpha), epidermal growth factor (EGF) and epidermal growth factor receptor (EGF-R) in 18 human ovaries.

Effect of motile sperm count after swim-up on outcome of intrauterine insemination.

Objective: To analyze the prognostic value of motile sperm count after swim-up in IUI with husband's sperm in a large group of subfertile couples.

Design: Retrospective study.

Setting: University hospital.

Sperm acrosome antigen-1, a molecule intimately involved in the regulation of the acrosome reaction: analysis of expression on spermatozoa from infertile couples.

Sperm acrosome antigen-1 (SAA-1) is a molecule on the acrosomal cap of sperm from the human and a number of mammalian and lower species. SAA-1 was initially characterized by a monoclonal antibody (mab) AG7 directed against SAA-1. Previous studies indicate that SAA-1 may play an important role in the regulation of the acrosome reaction in the human and other species.

[Idiopathic subfertility: pregnancy rate after IVF treatment in minimally stimulated monofollicular ovarian cycle].

Fifty-nine patients with idiopathic subfertility were treated with IVF in 176 minimally stimulated cycles. Twenty pregnancies were achieved, resulting in pregnancy rates of 11.4% per treatment cycle and 21.

[Clomiphene resistant PCO syndrome: IVF treatment as an alternative in failed gonadotropin treatment].

34 infertile patients with severe clomiphene-resistant polycystic ovary syndrome were treated with IVF/ET. Stimulation attempts with gonadotropins had resulted in multifollicular ovarian reactions, which did not allow standard ovulation induction using hCG due to the risk of multiple pregnancy. The resulting oocyte retrieval rates (87.

A three step protocol for the treatment of idiopathic subfertility.

650 couples with idiopathic subfertility (mean duration: 5.7 year, range 2-21 years) were treated during 2870 cycles by three assisted conception methods (each involving mild ovarian stimulation): I timed intercourse (TI), II intrauterine insemination (IUI). III in vitro fertilization/embryo transfer (IVF/ET).

Serum concentration and urinary excretion of "classical" estrogens, catecholestrogens and 2-methoxyestrogens in normal human pregnancy.

Catecholestrogens, 2-methoxyestrogens and "classical" estrogens (estrone, estradiol, estriol) were measured simultaneously in serum and urine samples of 220 pregnant women from the 8th week of pregnancy until to delivery. From these data we established the central 0.80 centile intervals as time specified reference intervals for each substance analyzed.

Urinary excretion of catecholestrogens, 2-methoxy-estrogens and "classical estrogens" throughout the normal menstrual cycle.

Catecholestrogens, 2-methoxyestrogens and "classical" estrogens (estrone, estradiol, estriol) were measured simultaneously in urine samples of healthy women during the menstrual cycle. All estrogen values reach a preovulatory maximum at the time of the LH peak and show a marked increase during the luteal phase as compared to the follicular phase. Catecholestrogens and estrone seem to behave similarly supporting the assumption that catecholestrogens are predominantly metabolites of estrone.

Familial 46,XY pure gonadal dysgenesis and gonadoblastoma/dysgerminoma: case report.

The case reports of two sisters admitted for evaluation of primary amenorrhea are presented. Gynecological and endocrinological investigations and chromosomal analysis led to the diagnosis of familial 46,XY gonadal dysgenesis. Both sisters underwent bilateral salpingo-oophorectomy and hysterectomy.

2-Hydroxylation and O-methylation of oestrogens by human placenta in vivo.

Concentrations of unconjugated and conjugated 2-hydroxyoestrogens and their 2-methylethers were determined by radioimmunoassay in serum obtained from the umbilical artery, umbilical vein, retroplacental space, and maternal peripheral vein at the time of delivery. The concentrations of unconjugated 2-hydroxyoestrogens and 2-methoxyoestrogens were found to be significantly higher in the post-placental vessels than in the pre-placental vessels. On the basis of the concentrations measured it was estimated that the placenta secretes about 1.