Antisense RNA Over-Expression Plus Antibacterial Dimethylaminohexadecyl Methacrylate Suppresses Oral Biofilms and Protects Enamel Hardness in Extracted Human Teeth.

() antisense RNA (AS) is a non-coding RNA that regulates cariogenic virulence and metabolic activity. Dimethylaminohexadecyl methacrylate (DMAHDM), a quaternary ammonium methacrylate used in dental materials, has strong antibacterial activity. This study examined the effects of AS on DMAHDM susceptibility and their combined impact on inhibiting biofilm formation and protecting enamel hardness.

Augmented Reality Head-Mounted Device and Dynamic Navigation System for Postremoval in Maxillary Molars.

Introduction: This study evaluates the feasibility of an augmented reality (AR) head-mounted device (HMD) displaying a dynamic navigation system (DNS) in the surgical site for fiber postremoval in maxillary molars and compares it to the DNS technique.

Methods: Fifty maxillary first molars were divided into 2 groups: AR HMD + DNS (n = 25) and DNS (n = 25). The palatal canal was restored with RelyX fiber post (3M ESPE) luted with RelyX Unicem (3M ESPE).

A Cadaver-based Comparison of Sleeve-guided Implant-drill and Dynamic Navigation Osteotomy and Root-end Resections.

Introduction: This study compared the accuracy and efficiency of fully guided static and dynamic computer-assisted surgical navigation techniques for osteotomy and root-end resection (RER).

Methods: Fifty roots from cadaver heads were divided into two groups: fully guided static computer-assisted endodontic microsurgery (FG sCAEMS) and dynamic computer-assisted endodontic microsurgery (dCAEMS) (all, n = 25). Cone-beam computed tomography scans were taken pre and postoperatively.

Comparison of a Novel Static Computer-aided Surgical and Freehand Techniques for Osteotomy and Root-end Resection.

Introduction: This study compared the accuracy and efficiency of a novel static computer-aided surgical technique using a 3-dimensional (3D)-printed surgical guide (3D-SG) with a fully guided drill protocol (3D-SG FG) to the freehand (FH) osteotomy and root-end resection (RER).

Methods: Forty-six roots from 2 cadaver heads were divided into 2 groups: 3D-SG FG (n = 23) and FH (n = 23). Cone-beam computed tomographic scans were taken preoperatively and postoperatively.

Novel dental resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate.

Objectives: White spot lesions (WSLs) are prevalent and often lead to aesthetic problems and progressive caries. The objectives of this study were to: (1) develop a novel resin infiltrant containing smart monomer dodecylmethylaminoethyl methacrylate (DMAEM) to inhibit WSLs, and (2) investigate the effects of DMAEM incorporation on cytotoxicity, mechanical properties, biofilm-inhibition and protection of enamel hardness for the first time.

Methods: DMAEM was synthesized using 1-bromododecane, 2-methylamino ethanol and methylmethacrylate.

The partial merger of two dolphin societies.

Interactions between mammalian social groups are generally antagonistic as individuals in groups cooperate to defend resources from non-members. Members of the family Delphinidae inhabit a three-dimensional habitat where resource defence is usually impractical. Here, we describe a long-term partial fusion of two communities of Atlantic spotted dolphins ().

Using social network analysis to confirm the 'gambit of the group' hypothesis for a small cetacean.

Social network analysis (SNA) can be used to explore a population's social structure and how individuals contribute to social cohesion. Quantifying relationships between individuals in a network can vary depending on the data available or the relationship of interest. Studies of readily visible species can use direct interaction measures in SNA, while studies of cryptic species usually rely on the 'gambit of the group'; individuals observed in a group are considered associates.

Projected shifts in loggerhead sea turtle thermal habitat in the Northwest Atlantic Ocean due to climate change.

It is well established that sea turtles are vulnerable to atmospheric and oceanographic shifts associated with climate change. However, few studies have formally projected how their seasonal marine habitat may shift in response to warming ocean temperatures. Here we used a high-resolution global climate model and a large satellite tagging dataset to project changes in the future distribution of suitable thermal habitat for loggerheads along the northeastern continental shelf of the United States.

Low-shrinkage-stress nanocomposite: An insight into shrinkage stress, antibacterial, and ion release properties.

The aims are: (a) To develop the first low-shrinkage-stress nanocomposite with antibacterial and remineralization capabilities through the incorporation of dimethylaminododecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP); (b) to investigate the effects of the new composite on biofilm inhibition, mechanical properties, shrinkage stress, and calcium (Ca) and phosphate (P) ion releases. The low-shrinkage-stress resin consisted of urethane dimethacrylate and triethylene glycol divinylbenzyl ether. Composite was formulated with 3% DMAHDM and 20% NACP.

S. mutans gene-modification and antibacterial resin composite as dual strategy to suppress biofilm acid production and inhibit caries.

Objective: Composite restorations are increasingly popular, but recurrent caries is a main reason for composite restoration failures. The objectives of this study were to investigate a dual strategy of combining rnc gene-deletion for Streptococcus mutans (S. mutans) with antibacterial dimethylaminohexadecyl methacrylate (DMAHDM) composite, and determine the effects of rnc gene-deletion alone, DMAHDM composite alone, and rnc-deletion plus DMAHDM composite, on biofilm growth and lactic acid production.

Ecosystem Model Skill Assessment. Yes We Can!

Need To Assess The Skill Of Ecosystem Models: Accelerated changes to global ecosystems call for holistic and integrated analyses of past, present and future states under various pressures to adequately understand current and projected future system states. Ecosystem models can inform management of human activities in a complex and changing environment, but are these models reliable? Ensuring that models are reliable for addressing management questions requires evaluating their skill in representing real-world processes and dynamics. Skill has been evaluated for just a limited set of some biophysical models.

Quantifying patterns of change in marine ecosystem response to multiple pressures.

The ability to understand and ultimately predict ecosystem response to multiple pressures is paramount to successfully implement ecosystem-based management. Thresholds shifts and nonlinear patterns in ecosystem responses can be used to determine reference points that identify levels of a pressure that may drastically alter ecosystem status, which can inform management action. However, quantifying ecosystem reference points has proven elusive due in large part to the multi-dimensional nature of both ecosystem pressures and ecosystem responses.

Methods for estimating spatial trends in Steller sea lion pup production using the Kalman filter.

Many species exhibit spatially varying trends in population size and status, often driven by differences among factors affecting individual subpopulations. Estimation and differentiation of such trends may be important for management, and a driving force for monitoring programs. The ability to estimate spatial differences in population trend may depend on assumptions regarding connectivity among subpopulations (stock structure or spatial overlap in stressors), information that is often poorly known.

Rapid cycling bipolar disorder in individuals with developmental disabilities.

The assessment and treatment of the rapid-cycling variant of bipolar disorder in individuals with developmental disabilities is challenging. Outcomes are optimized by a long-term commitment to the establishment of objective monitoring systems by interdisciplinary teams. In this retrospective case series, we contrast the phenomenology, clinical outcomes, treatment responses, and clinical characteristics of 26 individuals with bipolar disorder and developmental disabilities, 12 with nonrapid cycling courses and 14 with rapid cycling courses.

Written language production and neuropsychological function in children with traumatic brain injury.

Objective: To establish the early consequences of traumatic brain injury (TBI) on spontaneous written language production in children by examining writing deficits as a function of injury severity and correlating written performance with neuropsychological data.

Design: Case-control cohort study.

Setting: Two regional medical centers.

Predictors of family functioning and change 3 years after traumatic brain injury in children.

Objectives: To examine changes in family functioning from injury to 3 years after pediatric traumatic brain injury; to determine factors most predictive of family outcomes at 3 years and variables that promote positive outcomes and changes over time.

Design: Prospective cohort study.

Setting: Two regional tertiary care centers: cases followed for 3 years into community.

Neurobehavioral sequelae of severe pediatric traumatic brain injury: a cohort study.

Objectives: To determine: (1) the magnitude of neurobehavioral deficits following severe traumatic brain injury (TBI) in children, 3 weeks and 1 year after resolution of post-traumatic amnesia (PTA); (2) the relationship between deficits and injury severity; (3) the performance of cases compared to population norms versus individually matched controls.

Design: Prospective cohort study.

Setting: Two regional university medical centers.

Recovery trends over three years following pediatric traumatic brain injury.

This cohort study investigated the magnitude of neurobehavioral, academic, and "real world" deficits over the course of 3 years in children with mild, moderate, and severe traumatic brain injury (TBI), and their individually matched controls. This series of analyses addresses the history of recovery, tracing changes in performance over time. Data on 72 case-control pairs (mild, n = 40; moderate, n = 17; severe, n = 15) were available for three testing times: 3 weeks, 1 year, and 3 years after the resolution of post-traumatic amnesia.

Outcome of pediatric traumatic brain injury at three years: a cohort study.

This cohort study examined the neurobehavioral, academic, and "real world" consequences of mild, moderate, and severe traumatic brain injury in children at 3 years following the resolution of posttraumatic amnesia. Seventy-two children, aged 6 to 15 years at time of injury, were individually matched with controls on the basis of age, gender, school grade, and the classroom teacher's assessment of premorbid academic achievement and behavior. Both groups were assessed using the same standardized neuropsychological test battery and parent and teacher report measures as were used initially and at 1-year follow-up covering 10 cognitive, behavioral, and functional domains.

Family functioning and children's academic performance and behavior problems in the year following traumatic brain injury.

This study examined the roles of preinjury family and child functioning and injury severity in predicting 1-year outcomes and changes in academic performance and behavioral problems following childhood traumatic brain injury (TBI). Families of 94 children (ages 6 to 15) with TBI (mild = 50, moderate = 25, severe = 19) were consecutively enrolled from emergency departments of two regional medical centers. Standardized measures of family and child functioning and interviewer ratings were completed within 3 weeks of injury (measuring preinjury status), at 3 months, and 1 year.

Mild pediatric traumatic brain injury: adjusting significance levels for multiple comparisons.

The outcome following mild traumatic brain injury (TBI) is controversial. We addressed this topic in a study of neurobehavioural and 'real-world' functioning among 53 children with mild TBI and their matched controls, using statistical methods recently developed for multiple comparisons. Because the study involved calculation of 414 p-values, four methods of adjustment for multiple comparisons, including the Bonferroni method, were used to avoid 'false-positive' statistical significance.

Comparison of indices of traumatic brain injury severity as predictors of neurobehavioral outcome in children.

This study examined the ability of early measures of traumatic brain injury (TBI) severity to predict neurobehavioral and functional skill outcomes shortly after injury and at 1 year postinjury. Ninety-eight children aged 6 to 15 years with TBI were consecutively identified on presentation to two regional medical centers. Ten measures of TBI severity were subsequently administered: initial Glasgow Coma Scale (motor, eye, verbal, and total GCS), duration of loss of consciousness, Abbreviated Injury Scale Head score, GCS motor score at 3 days postinjury, days to reach a total GCS score of 15, days to reach a GCS motor score of six, and duration of posttraumatic amnesia (days to reach a 75% performance on the Children's Orientation and Amnesia Test [COAT]).

Family functioning and injury severity as predictors of child functioning one year following traumatic brain injury.

This study examined changes in children's functioning in the year following traumatic brain injury (TBI) and the preinjury family and injury factors most predictive of children's overall adaptive functioning and social competence at 1 year. Ninety-four children with TBI (mild = 50, moderate = 25, severe = 19) and their families were consecutively enrolled from two regional medical centers. The age range was from 6 years to 15 years.