Cu(II)-Mediated direct F-dehydrofluorination of phosphine oxides in high molar activity.

Background: The F/F-isotope exchange method employing P(V)-centered prosthetic groups demonstrates advantages in addressing mild one-step aqueous F-labeling of peptides and proteins. However, the molar activity (A) achieved through isotope exchange remains relatively low, unless employing a high initial activity of [F]F. To overcome this drawback, our work introduces a novel approach through a Cu-mediated direct F-dehydrofluorination of phosphine oxides.

BCAS2 Participates in Insulin Synthesis and Secretion via mRNA Alternative Splicing in Mice.

Insulin secreted by pancreatic β cells is essential for maintaining blood glucose levels. Diabetes is caused primarily by a loss of β cells or impairment of β-cell function. A previous whole-transcriptome analysis of islets from a type 2 diabetes group and a control group showed that a splicing disorder occurred in approximately 25% of splicing events.

Observation of near-inertial waves in the wake of four typhoons in the northern South China Sea.

Based on the velocity and temperature data recorded by two acoustic Doppler current profilers (ADCPs) at a mooring system deployed in the northern South China Sea (SCS), this study investigates the characteristics of near-inertial waves (NIWs) induced by typhoons Bebinca, Barijat, Mangkhut and Yutu in 2018. For the dynamical response, besides the motion of near inertial frequency induced by typhoons, the motion of 2 f ([1.80-2.

Qingrequzhuo capsule alleviated methionine and choline deficient diet-induced nonalcoholic steatohepatitis in mice through regulating gut microbiota, enhancing gut tight junction and inhibiting the activation of TLR4/NF-κB signaling pathway.

Qingrequzhuo capsule (QRQZ), composed of Morus alba L., Coptis chinensis Franch., Anemarrhena asphodeloides Bunge, Alisma plantago-aquatica subsp.

Development and comparison of Ga/F/Cu-labeled nanobody tracers probing Claudin18.2.

Claudin 18.2 (CLDN18.2) is an emerging target for the treatment of gastric cancers.

Clinical Utility of F-18 Labeled Fibroblast Activation Protein Inhibitor (FAPI) for Primary Staging in Lung Adenocarcinoma: a Prospective Study.

Purpose: The purpose of this study was to compare the primary staging of F-18 labeled fibroblast activation protein inhibitor ([F]F-FAPI) with that of F-18 labeled fluordesoxyglucose positron emission tomography/computed tomography ([F]F-FDG PET/CT) in patients with lung adenocarcinoma (LAD).

Procedures: We prospectively analyzed the images of LAD patients who underwent [F]F-FAPI and [F]F-FDG PET/CT between May 2020 and August 2021. [F]F-FAPI and [F]F-FDG uptakes were compared using the paired samples t test, and lesion numbers were compared using the Wilcoxon signed-rank test.

Increasing toll-like receptor 2 on astrocytes induced by Schwann cell-derived exosomes promotes recovery by inhibiting CSPGs deposition after spinal cord injury.

Background: Traumatic spinal cord injury (SCI) is a severely disabling disease that leads to loss of sensation, motor, and autonomic function. As exosomes have great potential in diagnosis, prognosis, and treatment of SCI because of their ability to easily cross the blood-brain barrier, the function of Schwann cell-derived exosomes (SCDEs) is still largely unknown.

Methods: A T10 spinal cord contusion was established in adult female mice.

Rb deficiency induces p21cip1 expression and delays retinal degeneration in rd1 mice.

Retinitis pigmentosa (RP) is a major cause of inherited blindness, and there is presently no cure for RP. Rd1 mouse is the most commonly used RP animal model. Re-expression of cell cycle proteins in post-mitotic neurons is considered an important mechanism of neurodegenerative diseases, including RP.

Immuno-HCR based on contact quenching and fluorescence resonance energy transfer for sensitive and low background detection of Escherichia coli O157:H7.

Escherichia coli O157:H7 makes a major threat to human health. Aiming to detect Escherichia coli O157:H7 sensitively, hybridization chain reaction signal amplified immunoassay (immuno-HCR) based on contact quenching (CQ) and fluorescence resonance energy transfer (FRET) was developed. The background of the new designed HCR hairpins (CQ-FRET hairpins) was reduced by contact-quenching fluorescein (FAM) and breaking FRET from donor (FAM) to acceptor (Cy5).

One-step F-fluorination of smart positron emission tomography tracer for sensing furin activity in tumors.

Introduction: Peptide analogues have attracted considerable attention in the field of developing novel positron emission tomography (PET) imaging agents due to their unique properties. Nevertheless, the complicated radiolabeling process and fast metabolism usually pose challenges to the clinical applications of peptide-based molecular probes. Herein a novel PET tracer containing a specific peptide sequence Arg-Val-Arg-Arg (RVRR), Acetyl-Arg-Val-Arg-Arg-Cys(StBu)-Gly(AMB[F]F)-CBT ([F]1), was designed and radiosynthesized using a simple and convenient one-step F-fluorination procedure.

Vitamin D receptor gene polymorphisms and the risk of colorectal cancer in a Chinese population.

Objective: The purpose was to investigate single nucleotide polymorphism of the vitamin D receptor gene and its possible relationship with colorectal cancer (CRC) in a Chinese population.

Methods: The vitamin D receptor (VDR) genotypes were determined by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) using endonuclease BsmI and FokI, and direct sequencing in 400 Chinese people, comprised of 200 CRC patients and 200 controls from the same area in China.

Results: The distribution of alleles (F/f) and genotypes (FF/Ff/ff) of the FokI had no significant difference between CRC patients and normal controls (P > 0.