Publications by authors named "F Wells"

Article Synopsis
  • Oxford Nanopore Technologies (ONT) has introduced R10 flowcells and V14 rapid sequencing chemistry, which were evaluated for sequencing Mycobacterium tuberculosis (Mtb) DNA from clinical samples, yielding mixed results in terms of quality and quantity.
  • In initial runs, sequencing generated a moderate amount of data (1.94 Gb, 214k reads), but only 49% of reads met acceptable quality standards (Phred score >8). Subsequent experiments with a clean-up step improved yield to 2.38 Gb and 66% quality, yet still fell short of optimal performance.
  • The study indicates that further optimization of ONT protocols is necessary, as the data did not meet the
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Background: With an increasing number of patients undergoing mitral valve repair, more patients are presenting for reoperation. This study aimed to evaluate factors influencing mortality and survival of patients undergoing reoperation for mitral valve surgery after previous mitral valve repair under a single surgeon.

Methods: We retrospectively collected data from 117 patients who underwent reoperation after previous mitral valve repair between 2010 and 2022.

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Microbial nanotechnology is an expanding research area devoted to producing biogenic metal and metalloid nanomaterials (NMs) using microorganisms. Often, biogenic NMs are explored as antimicrobial, anticancer, or antioxidant agents. Yet, most studies focus on their applications rather than the underlying mechanism of action or toxicity.

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Mycobacterium tuberculosis whole-genome sequencing (WGS) is a powerful tool as it can provide data on population diversity, drug resistance, disease transmission, and mixed infections. Successful WGS is still reliant on high concentrations of DNA obtained through M. tuberculosis culture.

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Vertical drop impacts of ferrofluids onto glass slides in a non-uniform magnetic field have been studied using high-speed photography. Outcomes have been classified based on the motion of the fluid-surface contact lines, and formation of peaks (Rosensweig instabilities) which affect the height of the spreading drop. The largest peaks are nucleated at the edge of a spreading drop, similarly to crown-rim instabilities in drop impacts with conventional fluids, and remain there for an extended time.

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