Nonaqueous reverse phase liquid chromatographic system for separation and quantitation of provitamins A.

A reverse phase liquid chromatographic (LC) system has been developed for separating the main naturally occurring carotenoids that have provitamin A activity. The system produces baseline separation of beta-carotene, alpha-carotene, and beta-cryptoxanthin (beta, beta-carotene-3-ol, 472-70-8) from biologically inactive zeinoxanthin (beta, epsilon-carotene-3-ol, 24480-38-4) and from a pigment believed to be alpha-cryptoxanthin (beta, epsilon-carotene-3'-ol). Some cis-isomers are also separated.

Arylsulfonate esters as hypocholesteremic agents: III. Mechanism of action studies.

The mechanism responsible for the hypocholestermic action of arylsulfonate esters of long chain fatty alcohols has been studied with rats fed either normocholesteremic or hypercholesteremic (1% cholesterol plus 0.5% glycoholate) diets. Linoleyl tosylate is more effective in lowering plasma and liver cholesterol levels of rats on the hypercholesteremic diet than several other hypocholesteremic agents tested.

Arylsulfonate esters of fatty alcohols as hypocholesterolemic agents. I: Oleyl and linoleyl p-toluenesulfonates.

Cholesterol levels of both plasma and liver of cholesterol-fed rats are lowered by feeding linoleyl and oleyl esters of p-toluenesulfonic acid. A structural specificity exists, since the effect is not observed with similar amounts of sodium p-toluenesulfonate nor with linoleyl methanesulfonate. Effective levels of these arylsulfonates do not alter liver to body weight ratios and maturation, nor reduce plasma cholesterol in normocholesterolemic rats.