Publications by authors named "F W NUSSBAUMER"
The synthetic neomycin-sensing riboswitch interacts with its cognate ligand neomycin as well as with the related antibiotics ribostamycin and paromomycin. Binding of these aminoglycosides induces a very similar ground state structure in the RNA, however, only neomycin can efficiently repress translation initiation. The molecular origin of these differences has been traced back to differences in the dynamics of the ligand:riboswitch complexes.
View Article and Find Full Text PDFUnlabelled: Several isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-N]-guanosine-5'-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcription. We showcase the utility of these labels to probe both structure and dynamics in two biologically important RNAs.
View Article and Find Full Text PDFArticle Synopsis
- N-methyladenosine (mA) is a chemical modification of RNA that influences gene expression by attracting specific proteins and impacts biochemical reactions, although the exact mechanisms remain unclear.
- Researchers used temperature-sensitive NMR techniques to show that mA pairs with uridine in a way that allows for rapid interconversion between stable and less stable forms, affecting RNA behaviors.
- Their findings suggest that mA significantly alters the speed of RNA processes like duplex annealing and hybridization, providing insights into how this modification can slow down various cellular functions.
Measuring the strength of the hydrogen bonds between DNA base pairs is of vital importance for understanding how our genetic code is physically accessed and recognized in cells, particularly during replication and transcription. Therefore, it is important to develop probes for these key hydrogen bonds (H-bonds) that dictate events critical to cellular function, such as the localized melting of DNA. The vibrations of carbonyl bonds are well-known probes of their H-bonding environment, and their signals can be observed with infrared (IR) spectroscopy.
View Article and Find Full Text PDF