Decoding the Challenges: navigating Intact Peptide Mass Spectrometry-Based Analysis for Biological Applications.

Quantitative analysis of peptides in biological fluids offers a high diagnostic and prognostic tool to reflect the pathophysiological condition of the patient. Recently, methods based on liquid chromatography coupled with mass spectrometry (LC-MS) for the quantitative determination of intact peptides have been replacing traditionally used ligand-binding assays, which suffer from cross-reactivity issues. The use of "top-down" analysis of peptides is rapidly increasing since it does not undergo incomplete or non-reproducible digestion like "bottom-up" approaches.

Custom GC×GC configuration for the selective isolation or removal of compounds from complex samples.

We developed a novel approach to selectively isolate or remove nearly any compound from complex mixtures of volatile organic compounds. This was achieved by customizing a GC×GC system with a Deans switch, a passive splitter, and a custom-made adapter for sample recollection. The new setup was evaluated with 106 standard chemicals covering a wide range of volatility (boiling points: 56 - 343 ⁰C) and polarity (log P: 0.

Laser-induced graphene-based aptasensor for the selective detection of Escherichia coli in urine samples.

A Laser-Induced Graphene-based (LIG) electrode covalently functionalized with an aptamer (P12-55) was used to develop an aptasensor detecting Escherichia coli in urine samples. Recent strides in material science have spotlighted LIG for exceptional attributes like robust mechanical resistance, superior conductivity, extensive surface area, and facile synthesis/patterning on various polymeric substrates. Variations in the aptasensor charge transfer resistance upon interaction with bacterial cells were evaluated by electrochemical impedance spectroscopy.

A MEPS-UHPLC-MS/MS analytical platform to detect isoprostanoids and specialized pro-resolving mediators in the urinary extracellular vesicles of mountain ultramarathon runners.

Oxylipins are powerful signalling compounds derived from polyunsaturated fatty acids (PUFAs) and involved in regulating the immune system response. A mass spectrometry-based method was developed and validated for the targeted profiling of 52 oxylipins (e.g.

Management of intra-abdominal infections: recommendations by the Italian council for the optimization of antimicrobial use.

Intra-abdominal infections (IAIs) are common surgical emergencies and are an important cause of morbidity and mortality in hospital settings, particularly if poorly managed. The cornerstones of effective IAIs management include early diagnosis, adequate source control, appropriate antimicrobial therapy, and early physiologic stabilization using intravenous fluids and vasopressor agents in critically ill patients. Adequate empiric antimicrobial therapy in patients with IAIs is of paramount importance because inappropriate antimicrobial therapy is associated with poor outcomes.