The control of risk in animal husbandry primary production.

This paper describes the nutritional aspects, including drinking water, that produce a negative influence on safety, health, nutritional and technological properties of food of animal origin, such as dairy and meat products, eggs, fish, and including processed and manufactured products. The purpose was to define an overview concerning the main aspects of food safety and to produce guidelines that best fit the different breeding systems, aiming to prevent health risk to consumers from fraudulent practices that should be avoided with the application of current rules in animal husbandry.

Determination of astaxanthin stereoisomers and colour attributes in flesh of rainbow trout (Oncorhynchus mykiss) as a tool to distinguish the dietary pigmentation source.

The presence of carotenoids in animal tissue reflects their sources along the food chain. Astaxanthin, the main carotenoid used for salmonid pigmentation, is usually included in the feed as a synthetic product. However, other dietary sources of astaxanthin such as shrimp or krill wastes, algae meal or yeasts are also available on the market.

Application of quantitative real-time PCR in the detection of prion-protein gene species-specific DNA sequences in animal meals and feedstuffs.

This study describes a method for quantitative and species-specific detection of animal DNA from different species (cattle, sheep, goat, swine, and chicken) in animal feed and feed ingredients, including fish meals. A quantitative real-time PCR approach was carried out to characterize species-specific sequences based on the amplification of prion-protein sequence. Prion-protein species-specific primers and TaqMan probes were designed, and amplification protocols were optimized in order to discriminate the different species with short PCR amplicons.