Publications by authors named "F T Hufert"

Article Synopsis
  • Rapid and precise detection of carbapenem resistance is crucial for effective infection control and antibiotic treatment.
  • The study focused on the challenges of isolating resistance genes from limited target cells while addressing PCR inhibitors present in synthetic stool samples.
  • Results showed that while qPCR detected resistance genes from culture fluid reliably, extraction methods struggled with synthetic stool, with reversed elution yielding the best results for DNA extraction.
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Objective: The aim of the study was to assess the current curricular status of content on infection prevention in hospitals during medical education prior to the development of a serious game on infection prevention in hospitals. In addition, the data collected was to be contrasted with the training for a specialist nurse in hygiene and infection prevention (FKHI).

Methodology: In an online survey, persons in charge of medical degree programs and continuing education centers for FKHI, SkillsLabs and professional associations in Germany were asked to answer 28 questions on framework conditions, teaching, examinations, and gamification.

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Nosocomial-associated diarrhea due to infection (CDI) is diagnosed after sample precultivation by the detection of the toxins in enzyme immunoassays or via toxin gene nucleic acid amplification. Rapid and direct diagnosis is important for targeted treatment to prevent severe cases and recurrence. We developed two singleplex and a one-pot duplex fluorescent 15 min isothermal recombinase polymerase amplification (RPA) assays targeting the toxin genes A and B ( and ).

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Article Synopsis
  • Healthcare workers faced a significant increase in depressive symptoms during the COVID-19 pandemic, influenced by high workload and stress from patient exposure.
  • The study analyzed data collected monthly from 166 staff at a German hospital, revealing that perceived stress consistently correlated with increased depression levels.
  • Specifically, medical professionals with direct COVID-19 exposure reported greater somatic depression symptoms, while administrators did not show the same trend.
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Background: Human coronaviruses are one of the leading causes for respiratory tract infections and for frequent primary care consultation. The human coronavirus NL63 (HCoV..

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