Publications by authors named "F Speleman"
Article Synopsis
- Neuroblastoma is a rare cancer found in kids that starts from cells that help develop nerves and is linked to 15% of cancer deaths in children.
- Researchers combined data from multiple studies on neuroblastoma to create a big map of 362,991 cells from 61 patients to learn more about the disease.
- This new cell atlas helps scientists understand how different cell types in the tumor work together and how they relate to treatment outcomes.
Neuroblastoma is a childhood developmental cancer; however, its embryonic origins remain poorly understood. Moreover, in-depth studies of early tumor-driving events are limited because of the lack of appropriate models. Herein, we analyzed RNA sequencing data obtained from human neuroblastoma samples and found that loss of expression of trunk neural crest-enriched gene associates with advanced disease and worse outcome.
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- Researchers have faced difficulties studying the early development of neural crest cells, which is crucial for understanding diseases like pediatric cancer neuroblastoma.
- To tackle this, they created a model using human pluripotent stem cells to mimic the normal development of sympathoadrenal cells, which can give rise to neuroblastoma.
- The study employed single-cell RNA sequencing to map gene expression changes during this development process, revealing a specific cell subpopulation marked by SOX2 that is associated with genetic mutations linked to neuroblastoma.
Neuroblastoma (NB) is the most common cancer in infancy with an urgent need for more efficient targeted therapies. The development of novel (combinatorial) treatment strategies relies on extensive explorations of signaling perturbations in neuroblastoma cell lines, using RNA-Seq or other high throughput technologies (e.g.
View Article and Find Full Text PDFIn biomedical research, high-throughput screening is often applied as it comes with automatization, higher-efficiency, and more and faster results. High-throughput screening experiments encompass drug, drug combination, genetic perturbagen or a combination of genetic and chemical perturbagen screens. These experiments are conducted in real-time assays over time or in an endpoint assay.
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