Multi-omics analysis reveals regime shifts in the gastrointestinal ecosystem in chickens following anticoccidial vaccination and challenge.

Coccidiosis, caused by parasites, significantly impacts poultry farm economics and animal welfare. Beyond its direct impact on health, infection disrupts enteric microbial populations leading to dysbiosis and increases vulnerability to secondary diseases such as necrotic enteritis, caused by . The impact of infection or anticoccidial vaccination on host gastrointestinal phenotypes and enteric microbiota remains understudied.

An Experimental Dermal Oedema Model for Apx Toxins of Actinobacillus pleuropneumoniae.

In-vivo models of Actinobacillus pleuropneumoniae (App) infection in pigs are required for the development of vaccines and investigations of pathogenicity. Existing models cause severe respiratory disease with pulmonary oedema, dyspnoea and severe thoracic pain, and careful monitoring and early intervention with euthanasia is, therefore, needed to avoid unnecessary suffering in experimental animals. As a potential replacement for the existing respiratory infection model, an in-vivo protocol was evaluated using intradermal or subcutaneous injection of different App strains and Apx toxins into the abdominal skin of pigs.

Differential expression of microRNAs in the caecal content and faeces of broiler chickens experimentally infected with .

Coccidiosis caused by spp. incurs significant morbidity and mortality in chickens, and is thus of great economic importance. intestinal lesion scoring remains one of the most common means of diagnosis; therefore alternative, non-invasive methods of diagnosis and monitoring would be highly desirable.

A Novel Whole Yeast-Based Subunit Oral Vaccine Against in Chickens.

Cheap, easy-to-produce oral vaccines are needed for control of coccidiosis in chickens to reduce the impact of this disease on welfare and economic performance. yeast expressing three antigens were developed and delivered as heat-killed, freeze-dried whole yeast oral vaccines to chickens in four separate studies. After vaccination, replication was reduced following low dose challenge (250 oocysts) in Hy-Line Brown layer chickens (p<0.

Impact of Oocyst Dose on Parasite Replication, Lesion Score and Cytokine Transcription in the Caeca in Three Breeds of Commercial Layer Chickens.

species parasites infect the gastrointestinal tract of chickens, causing disease and impacting on production. The poultry industry relies on anticoccidial drugs and live vaccines to control and there is a need for novel, scalable alternatives. Understanding the outcomes of experimental infection in commercial chickens is valuable for assessment of novel interventions.