Coexistence of Giant Hydatid Cyst and Hepatocellular Carcinoma: A Case Report and Literature Review.

From a global perspective, hepatocellular carcinoma (HCC) and hydatid cyst disease are both common; however, the endemic and zoonotic nature of hydatid cysts (due to larvae) makes the simultaneous detection of the two conditions a rare occurrence. In this case report, in a 43-year-old male patient, we aim to draw attention to the potential coexistence of HCC and liver hydatid cysts by presenting a case in which HCC tissue was detected in the cyst wall-removed by emergency surgery due to cyst perforation. Hydatid lesions in the liver may exhibit tumor-like growth characteristics.

On- and Off-Target Analyses of CRISPR-Cas12b Genome Editing Systems in Rice.

The CRISPR-associated Cas12b system is the third most efficient CRISPR tool for targeted genome editing in plants after Cas9 and Cas12a. Although the genome editing ability of AaCas12b has been previously investigated in rice, its off-target effects in plants are largely not known. In this study, we first engineered single-guide RNA (sgRNA) complexes with various RNA scaffolds to enhance editing frequency.

Boosting plant genome editing with a versatile CRISPR-Combo system.

CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided genome engineering in plants. However, these systems are mostly used separately, leaving their combinational potential largely untapped. Here we develop a versatile CRISPR-Combo platform, based on a single Cas9 protein, for simultaneous genome editing (targeted mutagenesis or base editing) and gene activation in plants.

Microbial composition of Kombucha determined using amplicon sequencing and shotgun metagenomics.

Kombucha, a fermented tea generated from the co-culture of yeasts and bacteria, has gained worldwide popularity in recent years due to its potential benefits to human health. As a result, many studies have attempted to characterize both its biochemical properties and microbial composition. Here, we have applied a combination of whole metagenome sequencing (WMS) and amplicon (16S rRNA and Internal Transcribed Spacer 1 [ITS1]) sequencing to investigate the microbial communities of homemade Kombucha fermentations from day 3 to day 15.

CRISPR-Cas nucleases and base editors for plant genome editing.

Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas) and base editors are fundamental tools in plant genome editing. Cas9 from (SpCas9), recognizing an NGG protospacer adjacent motif (PAM), is a widely used nuclease for genome editing in living cells. Cas12a nucleases, targeting T-rich PAMs, have also been recently demonstrated in several plant species.