Stepwise binary gradient high-performance liquid chromatographic system for routine drug monitoring.

Drug therapy is usually optimized by concentration measurement in patient serum. High-performance liquid chromatography (HPLC) is one of the most important analytical techniques used for therapeutic drug monitoring (TDM) of drugs for which no immunoassay kits are available. HPLC has been frequently used for screening purposes in toxicology, too.

Liquid chromatographic analysis of phenobarbital, phenytoin, and theophylline.

Sera from the routine of therapeutic drug monitoring were assayed for phenobarbital, phenytoin, and theophylline with three different methods: fluorescence polarization immunoassay as the standard procedure, the new CEDIA assays within a multicenter evaluation and HPLC which is known to yield results with a high specificity. CVs for between-day imprecision ranged from 2.6-8.

Decreased carbohydrate content of rat plasma apolipoproteins in galactosamine hepatitis.

The total carbohydrate content and the monosaccharide composition of plasma apolipoproteins isolated from normal and D-galactosamine-treated rats were investigated. In the lipoprotein classes VLDL, LDL and HDL of control animals, the carbohydrate content was 5,5%, 3,4% and 2,9% of dry weight apolipoprotein, respectively. 24 h after galactosamine treatment, these values dropped to 1.

[Gas chromatographic determination of di- and trisaccharides (author's transl)].

Three new methods are reported for the gas chromatographic analysis of di- and trisaccharides. Method I: Sugars are converted into their methoximes by reaction with methoxylamine hydrochloride in pyridine, followed by esterification with acetic anhydride. The separation is performed on OV 225 at 260 degrees C.