Publications by authors named "F S Pavone"
Article Synopsis
- The study demonstrates the use of Raman spectroscopy (RS) and surface-enhanced Raman spectroscopy (SERS) to measure intracellular cholesterol levels in human fibroblasts, critical for understanding cholesterol metabolism and diagnosing related disorders.
- SERS proved to be more sensitive and accurate in detecting cholesterol levels in fibroblasts from patients with type C Niemann-Pick disease compared to RS and traditional fluorescent methods.
- Researchers found that gold nanoparticles used in SERS were internalized by the cells and localized in lysosomes, enhancing the method's sensitivity and suggesting its potential for developing tools for screening and monitoring cholesterol-related diseases.
Article Synopsis
- Modern neuroscience aims to understand the complex connections among neuronal populations in the brain, a goal made feasible by recent technological advancements.
- New optical methods and genetically engineered indicators allow researchers to study and manipulate the activity of large groups of neurons, particularly in translucent larval zebrafish.
- The authors present a custom optical system that integrates advanced imaging and stimulation techniques, enabling them to noninvasively reconstruct neuronal connectivity in a specific brain region called the habenula.
Advanced 3D imaging techniques and image segmentation and classification methods can profoundly transform biomedical research by offering deep insights into the cytoarchitecture of the human brain in relation to pathological conditions. Here, we propose a comprehensive pipeline for performing 3D imaging and automated quantitative cellular phenotyping on Formalin-Fixed Paraffin-Embedded (FFPE) human brain specimens, a valuable yet underutilized resource. We exploited the versatility of our method by applying it to different human specimens from both adult and pediatric, normal and abnormal brain regions.
View Article and Find Full Text PDFAlthough Surface Enhanced Raman Scattering (SERS) is widely applied for ultrasensitive diagnostics and imaging, its potential is largely limited by the difficult preparation of SERS tags, typically metallic nanoparticles (NPs) functionalized with Raman-active molecules (RRs), whose production often involves complex synthetic approaches, low colloidal stability and poor reproducibility. Here, we introduce LipoGold Tags, a simple platform where gold NPs (AuNPs) clusters form via self-assembly on lipid vesicle. RRs embedded in the lipid bilayer experience enhanced electromagnetic field, significantly increasing their Raman signals.
View Article and Find Full Text PDFSignificance: Histopathological examination of surgical biopsies, such as in glioma and glioblastoma resection, is hindered in current clinical practice by the long time required for the laboratory analysis and pathological screening, typically taking several days or even weeks to be completed.
Aim: We propose here a transportable, high-density, spectral scanning-based hyperspectral imaging (HSI) setup, named HyperProbe1, that can provide , fast biochemical analysis, and mapping of fresh surgical tissue samples, right after excision, and without the need for fixing, staining nor compromising the integrity of the tissue properties.
Approach: HyperProbe1 is based on spectral scanning via supercontinuum laser illumination filtered with acousto-optic tunable filters.