Publications by authors named "F Ruiz Santiago"
Although cellular senescence has been recognized as a hallmark of aging, it is challenging to detect senescence cells (SnCs) due to their high level of heterogeneity at the molecular level. Machine learning (ML) is likely an ideal approach to address this challenge because of its ability to recognize complex patterns that cannot be characterized by one or a few features, from high-dimensional data. To test this, we evaluated the performance of four ML algorithms including support vector machines (SVM), random forest (RF), decision tree (DT), and Soft Independent Modelling of Class Analogy (SIMCA), in distinguishing SnCs from controls based on bulk RNA sequencing data.
View Article and Find Full Text PDFA comparison of the tablet-based and paper-based versions of the Aberdeen varicose vein questionnaire for quality-of-life assessment in patients with chronic venous disease.
J Vasc Bras
November 2024
Article Synopsis
- - The study evaluated the Aberdeen Varicose Vein Questionnaire (AVVQ), comparing paper and tablet versions to see how they scored chronic venous disease, how long they took to complete, and user-friendliness.
- - In testing with 88 patients, the tablet version was preferred for its ease of use and took less time to complete (4 minutes) compared to the paper version (9.5 minutes), with a significant difference in completion times (p<0.001).
- - Both versions produced similar AVVQ scores (p=0.431), suggesting that while the tablet saves time, both formats are equally effective in measuring the disease.
Blood coagulation is a vital physiological process involving a complex network of biochemical reactions, which converge to form a blood clot that repairs vascular injury. This process unfolds in three phases: initiation, amplification, and propagation, ultimately leading to thrombin formation. Coagulation begins when tissue factor (TF) is exposed on an injured vessel's wall.
View Article and Find Full Text PDFArticle Synopsis
- - Brazil’s gas stations operate with attendants filling fuel, exposing them to harmful substances known as BTEX, which can lead to serious health issues, including bone marrow degeneration and weakened immune systems.
- - This systematic review analyzed 1,086 articles on biomonitoring gas station workers, identifying 13 key studies that showcase advanced monitoring technologies like immunophenotyping and molecular cytogenetics, along with a focus on the health impacts of benzene exposure.
- - The review concludes that gas station workers are at risk and need ongoing health monitoring due to potential early signs of benzene-related toxicity, but there is no established standard for measuring health indicators effectively.
Senescence is a cell fate driven by different types of stress that results in exit from the cell cycle and expression of an inflammatory senescence-associated secretory phenotype (SASP). Here, we demonstrate that stable overexpression of miR-96-5p was sufficient to induce cellular senescence in the absence of genotoxic stress, inducing expression of certain markers of early senescence including SASP factors while repressing markers of deep senescence including LINE-1 and type 1 interferons. Stable miR-96-5p overexpression led to genome-wide changes in heterochromatin followed by epigenetic activation of p16, p21, and SASP expression, induction of a marker of DNA damage, and induction of a transcriptional signature similar to other senescent lung and endothelial cell types.
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