Publications by authors named "F Rubio Borrego"
Adoptive natural killer (NK) cell-based immunotherapy is a promising treatment approach in cancer that is showing notable efficacy against hematological malignancies. However, the success of NK cell immunotherapy in patients with solid tumors is limited due to several barriers, which include the immunosuppressive tumor microenvironment (TME), heterogeneity of tumor cells and poor NK cell infiltration into the tumor. Advances in 3D in vitro culture technologies have opened new avenues for the development of more physiological human cancer models that mimic important tumor features which are absent in traditional 2D studies and may be essential for the improvement of immunotherapies against solid tumors.
View Article and Find Full Text PDFSevere coronavirus disease 2019 (COVID-19) often leads to acute respiratory distress syndrome and multi-organ dysfunction, driven by a dysregulated immune response, including a cytokine storm with elevated proinflammatory cytokine levels. Natural killer (NK) cells are part of the innate immune system with a fundamental role in the defense against viral infections. However, during COVID-19 acute infection, they exhibit an altered phenotype and impaired functionality contributing to the immunopathogenesis of the disease.
View Article and Find Full Text PDFBackground: The APOE gene is the strongest genetic risk factor for late-onset Alzheimer's Disease (LOAD). However, the gene regulatory mechanisms at this locus remain incompletely characterized.
Methods: To identify novel AD-linked functional elements within the APOE locus, we integrated SNP variants with multi-omics data from human postmortem brains including 2,179 RNA-seq samples from 3 brain regions and two ancestries (European and African), 667 DNA methylation samples, and ChIP-seq samples.
Article Synopsis
- Renal injury is a common complication in autoimmune diseases like systemic lupus erythematosus (SLE), with around 20% of SLE patients developing lupus nephritis, which can lead to severe kidney failure.
- Research using animal models indicates that immune cell infiltration in the kidneys is associated with worsening kidney damage, suggesting that preventing this infiltration could help stop disease progression.
- The chapter outlines a detailed methodology for isolating and analyzing immune cells from kidney samples, specifically focusing on T cells and macrophages in a SLE mouse model, improving cell viability and consistency in experimental results.
Introduction: The APOE gene is the strongest genetic risk factor for late-onset Alzheimer's Disease (LOAD). However, the gene regulatory mechanisms at this locus have not been fully characterized.
Methods: To identify novel AD-linked functional elements within the locus, we integrated SNP variants with RNA-seq, DNA methylation, and ChIP-seq data from human postmortem brains.