Scale-up and impact of digital and molecular diagnostic technologies on TB diagnosis and timely linkage to care in Tajikistan.

Introduction: Tajikistan is scaling up molecular diagnostic and digital technologies to strengthen its fight against drug-resistant TB (DR-TB). The study aimed to document national scale-up GeneXpert/GxAlert and Open MRS from 2012-2019 and compare time taken from TB diagnosis to treatment and quality of data recording before and after the introduction of GxAlert.

Methodology: This was a longitudinal study that included a comparison of historical cohorts.

[Isolation of mRNA from protoplasts of spore-forming bacteria and study of its translation products].

Total RNA was isolated from the protoplasts of Bacillus intermedius 7P which actively produced alkaline exocellular RNAse. A fraction of polyadenylated RNA was isolated from this RNA using chromatography on poly(U) Sepharose. The total RNA and poly(A) +RNA were translated in Xenopus laevis oocytes.

[An intracellular inhibitory activity of RNase secreted by Bacillus intermedius].

Bacillus intermedius cells producing extracellular RNAse were found to contain its inhibitor and an RNAse-inhibitor complex. Bacillus subtilis and Escherichia coli cell lysates did not inhibit the activity of homogeneous extracellular RNAse produced by B. intermedius.

[Isolation and study of polyadenylated RNA from Bacillus intermedius].

A poly(A)+RNA fraction was isolated from the overall RNA of Bacillus intermedius using chromatography on poly(U) Sepharose and was shown to be electrophoretically heterogeneous. The presence of a polyadenylate segment was confirmed by hybridization with polyuridine. The biological activity of the poly(A)+RNA was proved by the translation in Xenopus laevis oocytes.

[Intracellular and extracellular ribonucleases of Bacillus intermedius].

Endocellular and exocellular ribonucleases were studied in Bacillus intermedius. Two fractions of ribonucleases (Rf 0.72 and 0.