Publications by authors named "F R Balkwill"
Single-cell RNA sequencing (scRNAseq) of tumour-infiltrating immune cells in high-grade serous ovarian cancer (HGSOC) omental biopsies reveals potential targets that could enhance response to neo-adjuvant chemotherapy (NACT). Analysis of 64,097 cells identifies NACT-induced overexpression of stabilin-1 (clever-1) on macrophages and FOXP3 in Tregs that is confirmed at the protein level. STAB1 inhibition in vitro induces anti-tumour macrophages.
View Article and Find Full Text PDFStudying cell behavior in live human tumors is crucial to understand and improve response to immunotherapies. Here, we present a protocol to slice human ovarian tumors ex vivo, maintain their viability for 24 h, and monitor the behavior of CD8 T and myeloid cells in real time. Furthermore, we detail procedures to semi-automatically analyze cell movements and aggregate and process behavior data.
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- Traditional in vitro testing of CAR T cells often overlooks the tumor microenvironment; thus, more complex models are needed to replicate real-life conditions.
- In studies using 3D human cell models, malignant cells showed resistance to CAR T cells due to faulty signaling, but co-culturing with fibroblasts improved CAR T cell effectiveness.
- A vascularized microfluidic device was created to mimic blood flow and tissue structure, enhancing CAR T cell function against cancer cells, and this research aims to speed up the development of better cancer therapies.
Past research has shown that growth mindset and motivational beliefs have an important role in math and science career interest in adolescence. Drawing on situated expectancy-value theory (SEVT), this study extends these findings by investigating the role of parental motivational beliefs (e.g.
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