Publications by authors named "F Pucci"
Recent thermodynamic and functional studies have been conducted to evaluate the impact of amino acid substitutions on Calmodulin (CaM). The Critical Assessment of Genome Interpretation (CAGI) data provider at University of Verona (Italy) measured the melting temperature (T) and the percentage of unfolding (%unfold) of a set of CaM variants (CaM challenge dataset). Thermodynamic measurements for the equilibrium unfolding of CaM were obtained by monitoring far-UV Circular Dichroism as a function of temperature.
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- - RNA-Puzzles is a collaborative project focused on improving the prediction of RNA three-dimensional structures, with predictions made by modeling groups before experimental structures are published.
- - A significant set of predictions was made by 18 groups for 23 different RNA structures, including various elements like ribozymes and aptamers.
- - The study highlights key challenges in RNA modeling, such as identifying helix pairs and ensuring proper stacking, and notes that some top-performing groups also excelled in a separate competition (CASP15).
The secondary metabolites of several plant species, particularly sesquiterpenic lactones (SLs) have been studied by different research groups for over 30 years. This group of metabolites presents numerous biological activities such as antibacterial, antiviral, antiulcer, cell proliferation inhibitor, and oocyte activator with participation in exocytosis processes. This study aims to assess some sperm parameters in epididymal gametes of exposed to increasing concentrations (0 to 2 mM) of DhL for various incubation times from 10 to 40 minutes.
View Article and Find Full Text PDFOptimal Operation of Cryogenic Calorimeters Through Deep Reinforcement Learning.
Comput Softw Big Sci
May 2024
Cryogenic phonon detectors with transition-edge sensors achieve the best sensitivity to sub-GeV/c dark matter interactions with nuclei in current direct detection experiments. In such devices, the temperature of the thermometer and the bias current in its readout circuit need careful optimization to achieve optimal detector performance. This task is not trivial and is typically done manually by an expert.
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- - This paper evaluates predictions for the "HMBS" challenge from the 2021 Critical Assessment of Genome Interpretation, focusing on how well participants predicted the effects of missense variants in the HMBS gene on yeast growth.
- - Despite using various algorithms, most predictors showed similar performance with correlation coefficients around 0.3, though some top predictors had a slightly better median correlation of ≥ 0.34 with experimental results.
- - Predictors were moderately effective in distinguishing between harmful and harmless variants, but overall accuracy remained low compared to experimental controls, highlighting a need for significant improvements in prediction methods, especially for variants in specific regions like the insertion loop.