Application of intracellular ATP determination in lymphocytes for HLA-typing.

The amount of adenosine triphosphate (ATP) in human lymphocytes was determined using a technique based on light emission from a bioluminescent reaction with luciferin-luciferase. The amount of ATP changed when cells were incubated in the presence of specific HLA antisera and complement. For determination of intracellular ATP a modified method was applied, which was based on reduction of extracellular ATP by the addition of ATPase.

Clinical experience in the treatment of complicated urinary tract infection with different doses of moxalactam (lamoxactam).

Moxalactam disodium underwent phase II evaluation to determine its efficacy, safety, and tolerance in the treatment of complicated urinary tract infections (UTI). Bacteriologic cultures of a clean midstream urine specimen were made and antibiotic sensitivities determined by FDA standard disk testing. Urinalysis and cultures were performed on specimens obtained within 24 h of starting therapy, on the 3rd or 4th day, and the last day of treatment, and again 5-9 days later.

Cefotetan in complicated urinary tract infections--clinical experience.

Cefotetan was evaluated to determine the efficacy and safety of a 1 g dose administered intravenously twice daily in the treatment of complicated urinary tract infections (UTI). Sixty patients entered and 58 patients completed the study. Duration of treatment ranged from 7 to 16 days with a mean of 10.

In vitro activity of N-formimidoyl-thienamycin in comparison to that of moxalactam and cefotaxime against gentamicin-resistant gram-negative bacteria.

The inhibitory and bactericidal activity of N-formimidoyl-thienamycin in vitro against 131 clinical isolates selected for their gentamicin resistance was compared with that of cefotaxime and moxalactam. All strains were inhibited by N-formimidoyl-thienamycin concentrations within a range of 0.12-4 mg/l.

Modulating effect of subinhibitory concentrations of compound LY 127935 on the virulence of Escherichia coli in the mouse.

The filamentous forms of a serum resistant Escherichia coli strain developed under in vitro exposure to subminimal inhibitory concentration of the compound LY 127935 showed a higher resistance against host defence than the nontreated control bacteria. Significantly diminished LD50 values and a longer lasting persistence in the peritoneal cavity of the mouse were found after intraperitoneal infection. In order to exclude the suggestion that the enhanced virulence was only due to the separation of filaments into normal virulent bacilli, the course of experimental infection with normal virulent germs was investigated under treatment with subtherapeutic doses of the antibiotic.