A microtiter plate assay for measurement of serum alanine aminotransferase in blood donors.

Background: Alanine aminotransferase (ALT) testing in blood donors is required in many countries as a surrogate test for viral hepatitis. Current automated ALT assays do not fulfill some operational requirements of blood banks.

Study Design And Methods: The standard ALT measurement method of the International Federation of Clinical Chemistry was adapted to the 96-well microtiter plate to obtain optimal conditions.