Straight talk.

Straight talk is a style of communication aimed at solving problems--without blaming, defending, bickering, or backbiting. Instead it uses assertiveness, openness, honesty, and collaboration to get the job done. Straight talk is built on the following skills.

A system for measuring the angular response of hemiparkinsonian monkeys to a food stimulus.

A Zenith Z-100 PC system was used to control an automated feeding system for determining the angular limits at which a monkey would respond to a food stimulus. Raisins secured to the end of a rotating arm by means of vacuum pressure were delivered to the monkey alternately from clockwise and counterclockwise directions at a fixed radius. The point at which the monkey took a raisin from the end of the arm was determined by an increase in negative pressure.

Design of flow chamber with electronic cell volume capability and light detection optics for multilaser flow cytometry.

A multibeam optical detection system has been developed with a high optical efficiency, achieved through a reduction in the number of optical interfaces employed in the system. This reduction is made possible by a combination of employing simple lenses, gluing the objective lens directly upon the face of the flow cuvette and the extraction of only one fluorescence signal from each laser beam. A modified flow chamber is also described that includes fluidic resistance elements for the elimination of most of the electric shielding normally associated with electronic cell volume measurements.

Quantitative microautoradiography of arteries: comparison of radioactivity to silver.

The local concentration of silver in developed aortic transmural microautoradiographs was compared to the corresponding 125I-labeled albumin radioactivity concentration [ci(x)] in the subjacent tissue. Silver [s(x)] was measured by electron probe X-ray microanalysis (EPA) and the corresponding ci(x) by direct gamma-ray counting. The results show 1) that the relationship between volume-averaged values of radioactivity (ci) and EPA signal (s) is adequately described by ci = Ks, where K is a proportionality constant, and 2) that ci(x) measured by EPA [i.