The POU transcription factor Oct-1 represses virus-induced interferon A gene expression.

Alpha interferon (IFN-alpha) and IFN-beta are able to interfere with viral infection. They exert a vast array of biologic functions, including growth arrest, cell differentiation, and immune system regulation. This regulation extends from innate immunity to cellular and humoral adaptive immune responses.

Purification and biochemical characterization of a novel cysteine protease of Entamoeba histolytica.

Cysteine proteases are important virulence factors of Entamoeba histolytica, the causative agent of amoebiasis. A novel cysteine protease from parasite extracts was purified 15-fold by a procedure including concanavalin A-Sepharose, hydroxylapatite and DEAE-Sepharose chromatography. The purification resulted in the obtainment of an homogeneous protein with a molecular mass of 66 kDa on native PAGE.

Purification and characterization of lactate dehydrogenase isoenzymes 1 and 2 from Molinema dessetae (Nematoda:Filarioidea).

High levels of lactate dehydrogenase (LDH; EC 1. 1. 1.

Enzymatic properties of the stonefish (Synanceia verrucosa Bloch and Schneider, 1801) venom and purification of a lethal, hypotensive and cytolytic factor.

The crude venom of the stonefish (Synanceia verrucosa) possesses numerous enzymatic properties (hyaluronidase, eight esterases and ten aminopeptidases). Verrucotoxin was isolated by DEAE and hydroxyapatite chromatography, followed by FPLC gel filtration on Superdex 200 HR 10/30. It was found to be a glycoprotein with a mol.