Publications by authors named "F Paglialunga"
Seismological observations highlighted that earthquakes are often followed by changes in elastic properties around the fault zone. Here, we studied the origin of these variations using stick-slip experiments on saw-cut granite samples presenting different degrees of bulk damage (i.e.
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- - Glucose 6-phosphate dehydrogenase (G6PD) is crucial for producing NADPH, especially during oxidative stress, and is vital for embryonic stem cell differentiation.
- - Research on G6pdDelta embryonic stem cells, which lack G6PD, reveals that they can differentiate into various cell types, like mesodermal cells and cardiomyocytes, but ultimately, their definitive erythrocytes undergo apoptosis after hemoglobin switching.
- - The apoptosis of these erythrocytes can be delayed with reducing agents or caspase inhibitors, but only restored G6PD activity can fully prevent this cell death, highlighting G6PD's essential role in blood cell development.
Glucose-6-phosphate dehydrogenase-deleted embryonic stem (ES) cells (G6pd Delta) proliferate in vitro without special requirements, but when challenged with oxidants fail to sustain glutathione disulphide reconversion to reduced glutathione (GSH), entering a condition of oxidative stress. Here, we investigate the signalling events downstream of GSH oxidation in G6pd Delta and wild-type (wt) ES cells. We found that G6pd Delta ES cells are very sensitive to oxidants, activating an apoptotic pathway at oxidant concentrations otherwise sublethal for wt ES cells.
View Article and Find Full Text PDFMouse embryonic stem (ES) glucose-6-phosphate (G6P) dehydrogenase-deleted cells ( G6pd delta), obtained by transient Cre recombinase expression in a G6pd -loxed cell line, are unable to produce G6P dehydrogenase (G6PD) protein (EC 1.1.1.
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