Publications by authors named "F Orzan"
Article Synopsis
- Recognizing the cause of cerebral ischemic events is crucial for effective treatment and prevention, yet there are no standard MRI criteria to identify an embolic etiology.
- This study aims to analyze the MRI characteristics of ischemic brain lesions that occur after transcatheter ablation of atrial fibrillation (AF) by systematically reviewing various studies.
- Results show that 17.2% of patients developed ischemic lesions post-ablation, primarily small cortical lesions under 10 mm, mostly found in the middle cerebral artery region, with a very low rate of symptomatic issues.
Article Synopsis
- The text discusses the limitations of traditional methods for deriving glioblastoma stem-like cells (GSCs), which do not maintain the diversity of these cells in lab models.
- It presents a new protocol that starts with whole tumor tissue to successfully grow heterogeneous GSC cultures in vitro.
- The document outlines steps for isolating, maintaining, and analyzing these GSCs, recommending reference to the work of De Bacco et al. for comprehensive guidance.
Glioblastoma (GBM) is known as an intractable, highly heterogeneous tumor encompassing multiple subclones, each supported by a distinct glioblastoma stem cell (GSC). The contribution of GSC genetic and transcriptional heterogeneity to tumor subclonal properties is debated. In this study, we describe the systematic derivation, propagation, and characterization of multiple distinct GSCs from single, treatment-naive GBMs (GSC families).
View Article and Find Full Text PDFIn colorectal cancer, the mechanisms underlying tumor aggressiveness require further elucidation. Taking advantage of a large panel of human metastatic colorectal cancer xenografts and matched stem-like cell cultures (m-colospheres), here we show that the overexpression of microRNA 483-3p (miRNA-483-3p; also known as MIR-483-3p), encoded by a frequently amplified gene locus, confers an aggressive phenotype. In m-colospheres, endogenous or ectopic miRNA-483-3p overexpression increased proliferative response, invasiveness, stem cell frequency, and resistance to differentiation.
View Article and Find Full Text PDFPurpose: Current glioma diagnostic guidelines call for molecular profiling to stratify patients into prognostic and treatment subgroups. In case the tumor tissue is inaccessible, cerebrospinal fluid (CSF) has been proposed as a reliable tumor DNA source for liquid biopsy. We prospectively investigated the use of CSF for molecular characterization of newly diagnosed gliomas.
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