Publications by authors named "F Naya-Catala"
Oxford Nanopore Technology (ONT) allows for the rapid profiling of aquaculture microbiomes. However, not all the experimental and downstream methodological possibilities have been benchmarked. Here, we aimed to offer novel insights into the use of different library preparation methods (standard-RAP and native barcoding-LIG), primers (V3-V4, V1-V3, and V1-V9), and basecalling models (fast-FAST, high-HAC, and super-accuracy-SUP) implemented in ONT to elucidate the microbiota associated with the aquatic environment and farmed fish, including faeces, skin, and intestinal mucus.
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- A confinement stress test was conducted to analyze the stress response in gilthead sea bream compared to European sea bass, focusing on behavioral changes using three-axis accelerometers.
- The study involved rearing gilthead sea bream at different stocking densities and monitoring various health metrics, showing that fish in high-density (HD) conditions had lower feed intake and growth rates, along with detectable muscle changes and minor injuries.
- After undergoing stress tests, fish in high-density settings demonstrated quicker recovery in activity and respiration, suggesting they became accustomed to the high stocking environment over time.
DNA methylation clocks provide information not only about chronological but also biological age, offering a high-resolution and precise understanding of age-related pathology and physiology. Attempts based on transcriptomic and epigenetic approaches arise as integrative biomarkers linking the quantification of stress responses with specific fitness traits and may help identify biological age markers, which are also considered welfare indicators. In gilthead sea bream, targeted gene expression and DNA methylation analyses in white skeletal muscle proved as a reliable marker of age-mediated changes in energy metabolism.
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- The study investigates how different types of aquafeeds (specifically various combinations of processed animal proteins and alternative ingredients) affect the growth and physiological health of farmed gilthead sea bream over a 77-day period.
- Four different diets were tested: a diet with processed animal protein (PAP), one without it (NOPAP), a mixed diet (MIX), and a commercial control (CTRL).
- Findings showed similar growth performance across all diets, but the NOPAP diet resulted in better markers for low oxidative stress and inflammation, while the PAP diet had poorer feed conversion and protein efficiency.
Differential Reshaping of Skin and Intestinal Microbiota by Stocking Density and Oxygen Availability in Farmed Gilthead Sea Bream (): A Behavioral and Network-Based Integrative Approach.
Microorganisms
July 2024
Fish were kept for six weeks at three different initial stocking densities and water O concentrations (low-LD, 8.5 kg/m and 95-70% O saturation; medium-MD, 17 kg/m and 55-75% O saturation; high-HD, 25 kg/m and 60-45% O saturation), with water temperature increasing from 19 °C to 26-27 °C. The improvement in growth performance with the decrease in stocking density was related to changes in skin and intestinal mucosal microbiomes.
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