Growth curves: a method of monitoring established long-term primary human tumor cell cultures for autologous immunotherapy.

Cytomorphologic methods evidentiate tumor cells (TC) and differentiate them from fibroblasts, but are not indicated f for long term cultures. Growth rate and curve shape of TC and autologous fibroblasts cultures were recorded and compared over 4 weeks. Both were morphologically documented ex vivo and after MGG stain.

Long-term primary human tumor cell cultures and mixed autologous tumor-lymphocyte cultures for adoptive specific antitumoral immunotherapy.

Anticancer immunotherapy may improve the body's cancer defences, quality of life and survival. Encouraging treatment models have been proposed, namely the non-specific LAK and ALT cell therapies; the relatively specific TIL/CTL cell therapy as well as allogenic cancer vaccines; autologous cancer vaccines. A procedure to prepare an autologous antitumor serum for cancer therapy using ex vivo activated autologous cells is here reported.

Higher survival of refractory metastatizing breast cancer after thermotherapy and autologous specific antitumoral immunotherapy.

46 women (average 54.3 yrs) with refractory metastatizing breast cancer were treated with thermotherapy and autologous specific immunotherapy (rhIL-2 ex vivo activated cells). Metastases involved one organ in 69%; in particular, bone, lung, liver.