Copper and Antimicrobial Residues in the Liver and Kidney-Antimicrobial Resistance and Cu Tolerance Unrelated in from Piglets' Faeces.

Antimicrobials, widely used in livestock, have induced the emergence of antimicrobial-resistant bacteria, prompting farmers to explore alternatives like copper. This study aims to determine antimicrobial residues and Cu concentrations in the liver and kidney of piglets and to investigate the correlation between Cu and antimicrobial use and the resistance to Cu and antimicrobials of isolated from piglets' faeces. Antimicrobial residues were quantified by UHPLC-ToF-MS; Cu was quantified using FAAS; microbiological methods were used for isolation, CuSO minimal inhibitory concentration (MIC), and antimicrobial susceptibility; and to detect genes, Real-Time PCR was used.

Hepatocyte nuclear factor 4-α is necessary for high fat diet-induced pancreatic β-cell mass expansion and metabolic compensations.

Aims: This study investigates the role of Hepatocyte Nuclear Factor 4α (HNF4α) in the adaptation of pancreatic β-cells to an HFD-induced obesogenic environment, focusing on β cell mass expansion and metabolic adaptations.

Main Methods: We utilized an HNF4α knockout (KO) mouse model, with CRE-recombinase enzyme activation confirmed through tamoxifen administration. KO and Control (CTL) mice were fed an HFD for 20 weeks.

Metabolic profiling and antibacterial activity of tree wood extracts obtained under variable extraction conditions.

Introduction: Tree bacterial diseases are a threat in forestry due to their increasing incidence and severity. Understanding tree defence mechanisms requires evaluating metabolic changes arising during infection. Metabolite extraction affects the chemical diversity of the samples and, therefore, the biological relevance of the data.

Knowledge of the nursing team about cardiac arrest and cardiopulmonary resuscitation: mixed methods studies.

Objective: To analyze the knowledge and perceptions of the nursing team about arrest and cardiopulmonary resuscitation in adults before and after in situ simulation in emergency care.

Method: A sequential explanatory mixed methods study conducted in an Emergency Care Unit. Quantitative data were obtained through pre- and post-simulation questionnaire answered by 21 professionals and analyzed using descriptive and inferential statistics.