Stylar peroxidase and incompatibility reactions in Petunia hybrida.

Self-, cross- and non-pollinated styles of Petunia hybrida were treated with 3-3'-diaminobenzidine for the ultrastructural localization of peroxidase activity. Wall peroxidases were absent in cross-pollinated styles, but they were detectable as an osmiophilic deposit on the cell walls of the outer portion of the transmitting tissue in self- and non-pollinated styles. The cell layer showing peroxidase activity was thicker in the self-pollinated styles than in the non-pollinated ones.

Immunological study on the wall proteins of different fruiting portions in Pleurotus ostreatus (Jacq. ex Fr.) Kummer and Agaricus bisporus (Lge.) Sing.

Immunological techniques are useful to clarify the sistematic scheme utilized for mushrooms. By comparing the various fruiting portions, a different composition in protein was observed in the cell wall of spores, pileus and stipe. The differences were present both in Agaricus bisporus and in Pleurotus ostreatus.

Cytoplasmic inheritance and ultrastructure of the male generative cell of higher plants.

The ultrastructure of the generative cell of the pollen grain has been studied in two different plants: Mirabilis jalapa L., where variegation is transmitted only by the egg cell, and Pelargonium zonale AIT., where variegation can be transmitted also by the pollen grain.