Publications by authors named "F Kiesewetter"
Article Synopsis
- 5-Hydroxymethylcytosine (5-hmC) plays a key role in DNA demethylation, and its levels are often disrupted in melanoma, complicating analysis due to lack of standardized methods.
- This study focused on analyzing 92 histological specimens of superficial spreading melanomas (SSM) and dysplastic nevi (DN) using digital pathology tools, specifically looking at the intensity and distribution of 5-hmC staining.
- Results indicated significant differences in 5-hmC-positive cell count between DN and SSM, with better diagnostic performance for assessing 5-hmC levels in the epidermis and junction, highlighting its potential role in differentiating these two conditions.
PRAME (PReferentially expressed Antigen in MElanoma) is a cancer testis antigen that is frequently expressed in melanoma compared to benign melanocytic proliferations and nevi. However, the interpretation of the intensity and distribution of PRAME immunostaining is not standardized a lot, which makes interpretation difficult. PRAME-stained histological slides of superficial spreading melanomas (SSM) and dysplastic nevi (DN) were digitized and analyzed using the digital pathology and image platform QuPath.
View Article and Find Full Text PDFTo facilitate the recovery process of chronic and hard-to-heal wounds novel pro-resolving treatment options are urgently needed. We investigated the pro-regenerative properties of soluble CD83 (sCD83) on cutaneous wound healing, where sCD83 accelerated wound healing not only after systemic but also after topical application, which is of high therapeutic interest. Cytokine profile analyses revealed an initial upregulation of inflammatory mediators such as TNFα and IL-1β, followed by a switch towards pro-resolving factors, including YM-1 and IL-10, both expressed by tissue repair macrophages.
View Article and Find Full Text PDFGenomic AZFb deletions in Yq11 coined "classical" (i.e. length of Y DNA deletion: 6.
View Article and Find Full Text PDFBackground: The assessment of tumor size by RECIST using CT scans and MRIs is considered to be standard of care for staging cancer patients. Despite radiologic evidence of widespread disease, we document for the first time that patients were completely free of viable tumor.
Case Presentation: Two patients with metastatic melanoma were treated with immune checkpoint inhibitors (ipilimumab/ nivolumab) and progressive metastases were detected on CT-scans performed shortly before histologic examinations.