The radioimmunological dosage of human prolactin using the hormone as tracer and reference and an anti-prolactin antiserum is described. The labeling of human prolactin is carried out in the presence of a small quantity of chloramine T (10 mug). The purification of the labeled hormone is carried out by gel filtration on Sephadex G-75 or by polyacrylamide gel electrophoresis.
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November 1971