Ultrastructure of Candida ingens: a yeast that can assimilate volatile fatty acids.

A defined liquid medium was developed for culture of Candida ingens on either volatile fatty acids or glucose as carbon source. Buffering capacity at pH 5.4 was achieved by inclusion of 50 mM phthalic acid which is not assimilated.

Electron cytochemical demonstration of the capsule of yeast-like Sporothrix schenckii.

Staining of yeast-like cells of Sporothrix schenckii by the cationic dye Alcian blue 8GX and lanthanum nitrate permitted the demonstration in thin sections of an electron opaque material localized at and along the outermost wall surface. This material was associated with the characteristic microfibrillar layer of the external cell wall. It was absent or largely removed when cells were grown in shaken liquid culture or after washing of cells from static cultures.

Fine structural studies of the Chrysosporium state of Renispora flavissima.

Aspects of the fine structural characteristics of conidial and hyphal cells of the imperfect Chrysosporium state of the newly reported gymnoascaceous fungus, Renispora flavissima, are described and illustrated by electron micrographs of ultrathin sections. Preservation of the substructure of the thick-walled, smooth to tuberculate conidia was best achieved after fixation in permanganate. The conidial wall consisted of two conspicuous zones (or layers) of varying thickness, texture, and electron opacity.

Fine structural studies of Rhodococcus species.

Fine structural aspects of Rhodococcus rhodochrous and R. equi are described and illustrated by electron micrographs after staining of cells by a variety of electron cytochemical procedures. The cell contents of these actinomycetous bacteria were those of a typical prokaryotic cell and consistent with that observed for other species of the Actinomycetales.