[Transcriptional factor Xanf1 interacts with the focal adhesion protein zyxin in the early development of the Xenopus laevis brain].

We searched for potential protein partners of the homeodomain transcription repressor Xanf1, a key transcriptional regulator of the early development of the forebrain, using a yeast two-hybrid system and identified for the first time the LIM domain protein zyxin from the African clawed frog Xenopus laevis. This protein is interesting because it can play the role of a signal transmitter from cell receptors to the cell nucleus and, thus, participate in the regulation of cell morphogenetic movements and gene expression. The interaction of zyxin with Xanf1 was confirmed by the immunoprecipitation of a complex of endogenous zyxin with the hybrid myc-Xanf1 protein from the lysate of X.

[Interaction of the molecular motors].

A review addresses the up-to-date evidence on the regulation of the organelle transport along microtubules in a very specific aspect of the interaction of the molecular motors of the opposite directions.

[Study of the mitochondria movement using videomicroscopy].

A stable cell line CV-1 was obtained for vital observation of the transport of mitochondria in animals cells, which express a fragment of the resident protein of mitochondria marked by yellow fluorescent protein. The parameters and conditions of movement of the mitochondria in living cells were established using fluorescence videomicroscopy. Under the normal conditions, only a small part of mitochondria (ca.