Microgametophyte Development in L. and First Androgenesis Induction Through Microspore Embryogenesis.

Development of double haploids is an elusive current breeding objective in L. We have studied the whole process of anther and pollen grain formation during meiosis, microsporogenesis, and microgametogenesis and correlated the different microgametophyte developmental stages with bud length in plants from varieties USO31 and Finola. We also studied microspore and pollen amyloplast content and studied the effect of a cold pretreatment to excised buds prior to microspore culture.

Development of a Direct Plant Regeneration Protocol From L. Seedling Explants: Developmental Morphology of Shoot Regeneration and Ploidy Level of Regenerated Plants.

shoot regeneration can efficiently contribute to the improvement of recalcitrant L. We aimed at developing a highly efficient protocol for direct regeneration of plants from different explants (cotyledon, hypocotyl, and true leaf) from seedlings of monoecious short-day varieties Ferimon, Felina32, Fedora17, and USO31, together with dioecious neutral-day variety Finola. Ten regeneration media, including already published protocols, and self-designed combinations of plant growth regulators were tested.

Development and Genetic Characterization of Advanced Backcross Materials and An Introgression Line Population of in a Background.

Advanced backcrosses (ABs) and introgression lines (ILs) of eggplant () can speed up genetics and genomics studies and breeding in this crop. We have developed the first full set of ABs and ILs in eggplant using , a wild eggplant that has a relatively high tolerance to drought, as a donor parent. The development of these ABs and IL eggplant populations had a low efficiency in the early stages, because of the lack of molecular markers and genomic tools.

The first de novo transcriptome of pepino (Solanum muricatum): assembly, comprehensive analysis and comparison with the closely related species S. caripense, potato and tomato.

Background: Solanum sect. Basarthrum is phylogenetically very close to potatoes (Solanum sect. Petota) and tomatoes (Solanum sect.

Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells.

Within nanotechnology, gold and silver nanostructures have unique physical, chemical, and electronic properties [1,2], which make them suitable for a number of applications. Moreover, biosynthetic methods are considered to be a safer alternative to conventional physicochemical procedures for both the environmental and biomedical applications, due to their eco-friendly nature and the avoidance of toxic chemicals in the synthesis. For this reason, employing bio routes in the synthesis of functionalized silver nanoparticles (FAgNP) have gained importance recently in this field.