Publications by authors named "F J Varga"
Data and Process Harmonisation of Multi-National, Multi-Site Research Data.
Stud Health Technol Inform
August 2024
To achieve a single fully harmonised research data set suitable for analysis from data collected at multiple sites requires not only semantic integration of collection concepts and convergence onto single collection units, but harmonisation of data collection processes. We describe our experience of identifying harmonisation challenges in the Precision ALS project, with particular focus on process alignment challenges in a multi-site multi-national research data collection project.
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- The study examines the synthesis and variation of pyrethrin, an insecticidal compound from Dalmatian pyrethrum, across 15 natural populations in the eastern Mediterranean.
- Researchers implemented a controlled field experiment to analyze pyrethrin content, aiming to attribute differences to genetic adaptations influenced by specific bioclimatic conditions.
- Results revealed significant variation in pyrethrin levels among populations, with the highest concentrations found in Budva and Podgorica, and five distinct bioclimatic groups formed based on environmental factors affecting pyrethrin composition.
Article Synopsis
- - Simple sequence repeats (SSRs) are widely used molecular markers important for conservation genetics, population studies, and genetic mapping, benefiting from advances in next-generation sequencing and bioinformatics tools for their design.
- - A new standalone pipeline has been developed to design PCR primers for microsatellite loci using multiple genomic assemblies from different species, assessing their polymorphism through in silico testing.
- - This approach eliminates the need for labor-intensive lab tests, producing efficient and promising SSR markers that can help identify interspecies hybrids, with demonstrated success across various taxonomic levels.
Article Synopsis
- The research explores the relationship between chloroplast genome lengths and the taxonomic proximity of species, based on the assumption that closely related species will have similar genome sequences.
- The study focuses on sequences from the asterid and rosid clades, finding that chloroplast length distributions are generally narrow within families and genera.
- Factors causing length variations include parasitic lifestyles, changes in the inverted repeat regions, and polyphyletic relationships, while outliers in length distributions suggest potential errors in sequence assembly.
Saprolegnia parasitica causes saprolegniosis, a disease responsible for significant economic losses in aquaculture and declines of fish populations in the wild, but the knowledge of its distribution and prevalence in the environment is limited. We developed a fast, sensitive and specific S. parasitica droplet digital PCR (ddPCR) assay and demonstrated its applicability for the detection and quantification of the pathogen in environmental samples: swab DNA collected from the host (trout skin, surface of eggs) and environmental DNA extracted from water.
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